LOCUS       sequence-71741-e        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  TITLE     Series-13: Co-transformation vectors for Series-2 transfer vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET His6 TEV co-transformation cloning vector
            ...
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(2..79)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     125..144
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        125..143
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             232..254
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             274..291
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             292..312
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(437..455)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      451..498
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
       61 tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg
      121 aaattaatac gactcactat agggagacca caacggtttc cctctagtgc cggctccgga
      181 gagctcttta attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa
      241 ctttaagaag gagatataca tatgaaatct tctcaccatc accatcacca tgaaaacctg
      301 tacttccaat ccaatattgg aagtggataa cggatccgcg atcgcggcgc gccacctggt
      361 ggccggccgg taccacgcgt gcgcgctgat ccggctgcta acaaagcccg aaaggaagct
      421 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
      481 gtcttgaggg gttttttgct gaaacctcag gcatttgaga agcacacggt cacactgctt
      541 ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta acgaccctgc
      601 cctgaaccga cgacaagctg acgaccgggt ctccgcaagt ggcacttttc ggggaaatgt
      661 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgaa
      721 ttaattctta gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat
      781 tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc
      841 agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa
      901 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag
      961 tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttcc
//
