LOCUS       sequence-71744-e        1056 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1056)
  TITLE     Series-13: Co-transformation vectors for Series-2 transfer vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET His6 StrepII TEV co-transformation cloning...
FEATURES             Location/Qualifiers
     source          1..1056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(2..79)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     125..144
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        125..143
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             232..254
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             274..291
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             307..330
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             337..357
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(482..500)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      496..543
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
       61 tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg
      121 aaattaatac gactcactat agggagacca caacggtttc cctctagtgc cggctccgga
      181 gagctcttta attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa
      241 ctttaagaag gagatataca tatgaaatct tctcaccatc accatcacca tggttcttct
      301 gctagctgga gccacccgca gttcgaaaaa ggcgccgaaa acctgtactt ccaatccaat
      361 attggaagtg gataacggat ccgcgatcgc ggcgcgccac ctggtggccg gccggtacca
      421 cgcgtgcgcg ctgatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
      481 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
      541 ttgctgaaac ctcaggcatt tgagaagcac acggtcacac tgcttccggt agtcaataaa
      601 ccggtaaacc agcaatagac ataagcggct atttaacgac cctgccctga accgacgaca
      661 agctgacgac cgggtctccg caagtggcac ttttcgggga aatgtgcgcg gaacccctat
      721 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgaattaat tcttagaaaa
      781 actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt
      841 tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg
      901 caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt
      961 tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg
     1021 gtgagaatgg caaaagttta tgcatttctt tccaga
//
