LOCUS       sequence-71926-e         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant protein production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Posner M, Kiss AJ, Skiba J, Drossman A, Dolinska MB, Hejtmancik JF, 
            Sergeev YV
  TITLE     Functional validation of hydrophobic adaptation to physiological 
            temperature in the small heat shock protein alphaA-crystallin.
  JOURNAL   PLoS One. 2012;7(3):e34438. doi: 10.1371/journal.pone.0034438. Epub 
            2012 Mar 29.
  PUBMED    22479631
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3):e34438. doi"; pages: " 
            10.1371/journal.pone.0034438. Epub 2012 Mar 29"
COMMENT     Sequence Label: Danio rerio cryaba
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             569..586
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(639..657)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      653..700
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gaaggagata tacatatgga aatttctatc cagcatccct ggtatcgccg acccttgttc
       61 ccaggcttct tcccttatcg aatttttgat cagtattttg gagagcatct atctgacagt
      121 gacccttttt cacctttcta caccatgttt tactatcgtc cttatttgtg gcgctttcca
      181 agctggtggg acagtggcat gtcggagatg agacaggata gagatcgctt tgtaatcaac
      241 ctggatgtga agcacttctc tccagatgaa ctgactgtca aggtcaatga ggattttatt
      301 gagattcatg gaaaacatga tgaacggcag gatgatcacg gcatagtggc gagggagttt
      361 ttcaggaagt acaagatccc tgccggtgta gatccagggg ccatcacctc ttctctatcc
      421 tctgacggcg tgttgaccat caacacactt cgccatcagc tggacatcct tgaacgcagc
      481 atccctatca tctgtggaga gaaaccacca gcacagaaat gaggatccga attcgagctc
      541 cgtcgacaag cttgcggccg cactcgagca ccaccaccac caccactgag atccggctgc
      601 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
      661 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
      721 cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
      781 acgcgcagcg tgaccgctac acttgccagc gcccta
//
