LOCUS       sequence-72005-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET reporter for Ca2+ inside primary cilia.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Su S, Phua SC, Derose R, Chiba S, Narita K, Kalugin PN, Katada T, 
            Kontani K, Takeda S, Inoue T
  TITLE     Genetically encoded calcium indicator illuminates calcium dynamics 
            in primary cilia.
  JOURNAL   Nat Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647.
  PUBMED    24056873
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647."
COMMENT     Sequence Label: TNXXL-CTS20
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(104..616,617..619,620..622))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     297..316
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     556..577
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(683..704)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggccgccact gtgctggata tcctgcagtt attctggttt tatttttctg gttttgcttt
       61 tcttaaacca gcagcaaacg agacagctca ggaattcata gtcctcgatg ttgtggcgga
      121 tcttgaagtt ggccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc
      181 tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
      241 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
      301 tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg
      361 cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcgggcgaag cacatcaggc
      421 cgtagcccag ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga
      481 tgaacttcag ggtcagcttg ccgttggtgg catcgccctc gccctcgccg cgcacgctga
      541 acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca
      601 gctcctcgcc cttgctcacc atgctgccgc cggtaccgcc cttgtacagc tcgtccatgc
      661 cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt
      721 tggggtcttt gctcagcttg gactggtagc tcaggtagtg gttgtcgggc agcagcacgg
      781 ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg c
//
