LOCUS       sequence-72074-e         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length RON3 ectodomain
            with no N-linked glycans upon cotransfection with BirA in mammalian 
            cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: RON3-bio
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      781..790
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(813..832)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 cctcactctt ataggccgtg atggaggtcg acggcgcgcc gttcatgatc agcttatcga
       61 agctggccac gtggttgaac acgtaggcgt tggtgaagca caggtcgatc agcttgtggt
      121 tgatcaggcc gatgatgccc cgcaggctct gcacgaagga cacgctcagg aaggttctgg
      181 ccagcagcag gccatccttc ttcagggcgt ccatcacgct ccaatagctg tcgctcttgg
      241 ccttcttgta caccaggatg gcctggttca tctcgttctt cacggtgtcg tcgatctcga
      301 agttggggta gtcgaactcg ctgatcaggt acttcttgaa cacgttgaac ttccgcttgt
      361 tgctgctccg ctcggccttg ctgatgatct tgacgatctt cttcacgttt ttcttgttca
      421 ggatggtcag gtagaagaag aagttggtcc ggctgaagct ctcgtacatg gccacgttct
      481 tgtggaagta cacgttcagg tggaagcccc ggtacagcag ggtgttgatc agcttggcca
      541 cgtagtactg gttgaagatg ctgctggcct tcaggctcag aaagctgtac tcgttgtggt
      601 tgctgtcctg gttgatctcg atgtggtgct tgcactggat gtcctttttc ttgaagtact
      661 ggccgatgtc gttgttgtac acggccaggt tcttcacttt gttattgttg ttctcgatct
      721 tgttggcggc cgcaccagac atgcagagca gcagggacat gagtacctgg gtctgaaact
      781 ccatggtggc aaacttggac ctgggagtgg acacctgtgg agagaaaggc aaagtggatg
      841 tcattgtcac tcaagtgtat ggccagatcg ggccaggtga atatcaaatc ctcctcgttt
      901 ttggaaactg acaatcttag cgca
//
