LOCUS       sequence-72080-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length MSP5 ectodomain
            with no N-linked glycans upon cotransfection with BirA in mammalian 
            cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: MSP5-bio
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      787..796
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(819..838)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 cctcactctt ataggccgtg atggaggtcg acggcgcgcc agagttcagg atgatgcact
       61 tggtgcccag cagatagccg tccttgcaga tgcacttcac gccgatgttg ttgatgttga
      121 tgcagatctg gtcgtcgctg cagccgccgt tgttgatggc gcaggacttc cggttctggt
      181 cgctcatcat gttgtacagc agcatctcat cggccacgtt cttctccacc ttgttgtacc
      241 gcagggcgct gtagtgtggc acctcgtcgt ccacgttgtc gaagtgcagc cggttggggg
      301 gttcctggcc gatctcggcg ttctcgttga tttccaggtc cagtggttcc tcgccgtttt
      361 cctcttttgt ctcaggattg ttgctattgt gctcgctgat gtccacgctt ctggtggcgc
      421 tctcggcgct attctcgctg ctgtccaggt tgggattgct gttctctttt tcctcgggct
      481 ctttctggat gttctgggcg ttgttggagt tgctcttgct ctcgtcgtgg ctgatgtcgt
      541 tgggctgctt gtcggcgctg ttgctgttct tctcgttcat cttggtgggc aggctgcctt
      601 cctctttcag ctcattctcg ttgttccggt tgttgttgcc gttctctttg ttcttgctgg
      661 tgaagccgcc ctcgcccttc tcatcgttca tcagccgccg cacgaccagg aagttctcgt
      721 tcttgttgtt ggcggccgca ccagacatgc agagcagcag ggacatgagt acctgggtct
      781 gaaactccat ggtggcaaac ttggacctgg gagtggacac ctgtggagag aaaggcaaag
      841 tggatgtcat tgtcactcaa gtgtatggcc agatcgggcc aggtgaatat caaatcctcc
      901 tcgtttttgg aaactgacaa tctta
//
