LOCUS       sequence-72128-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for Ca2+ inside primary cilia.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Su S, Phua SC, Derose R, Chiba S, Narita K, Kalugin PN, Katada T, 
            Kontani K, Takeda S, Inoue T
  TITLE     Genetically encoded calcium indicator illuminates calcium dynamics 
            in primary cilia.
  JOURNAL   Nat Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647.
  PUBMED    24056873
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647."
COMMENT     Sequence Label: 5HT6-GCaMP5G
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(67..88)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(328..347)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cagggcggta ccggagggag catggtgagc aagggcgagg agctgttcac cggggtggtg
       61 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      121 ggtgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      181 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
      241 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      301 atccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      361 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      421 gacggcaaca tcctggggca caagctggag tacaacctgc cggaccaact gactgaagag
      481 cagatcgcag aatttaaaga ggctttctcc ctatttgaca aggacgggga tgggacaata
      541 acaaccaagg agctggggac ggtgatgcgg tctctggggc agaaccccac agaagcagag
      601 ctgcaggaca tgatcaatga agtagatgcc gacggtgacg gcacaatcga cttccctgag
      661 ttcctgacaa tgatggcaag aaaaatgaaa tacacagaca gtgaagaaga aattagagaa
      721 gcgttccgtg tgtttgataa ggatggcaat ggctacatca gtgcagcaga gcttcgccac
      781 gtgatgac
//
