LOCUS       sequence-72229-e         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  TITLE     Jorgensen unpublished mosSCI reagents
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCFJ210
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(191..295)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     501..523
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     515..532
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     516..532
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
       61 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
      121 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
      181 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
      241 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
      301 ttccccgaaa agtgccacct gacgtagtta acaaaaaaaa gcccgccgaa gcgggcttta
      361 ttaccaagcg aagcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
      421 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
      481 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gttgatcagt
      541 catccaagtg gggatcagga agaagttgat aaagtgattc aaaaactcga ggagctcaac
      601 acagcgatga acccaatttt gagagcaaca ctgacgaaga actatgacgc gagggtaggc
      661 actaccttga agctatccac ctgcctgcct gccgcctatt tccatgtgca gtaggaacgt
      721 gtgtaggtat aggcag
//
