LOCUS       sequence-72257-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination plasmid with mClover for Golden Gate reaction of TALE 
            assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Miyanari Y, Ziegler-Birling C, Torres-Padilla ME
  TITLE     Live visualization of chromatin dynamics with fluorescent TALEs.
  JOURNAL   Nat Struct Mol Biol. 2013 Oct 6. doi: 10.1038/nsmb.2680.
  PUBMED    24096363
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2013 Oct 6. doi: 10.1038/nsmb.2680."
COMMENT     Sequence Label: pTALYM3
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(25..44)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(26..44)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(51..68)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(51..67)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(60..82)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      208..663
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(295..314)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     505..526
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(730..749)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccccgggtac cgagctcgaa ttcgccctat agtgagtcgt attacaattc actggccgtc
       61 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
      121 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
      181 cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg
      241 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
      301 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
      361 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
      421 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
      481 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
      541 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
      601 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
      661 tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
      721 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaaa
      781 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agaca
//
