LOCUS       sequence-72280-e         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination plasmid with mClover for Golden Gate reaction of TALE 
            assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Miyanari Y, Ziegler-Birling C, Torres-Padilla ME
  TITLE     Live visualization of chromatin dynamics with fluorescent TALEs.
  JOURNAL   Nat Struct Mol Biol. 2013 Oct 6. doi: 10.1038/nsmb.2680.
  PUBMED    24096363
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2013 Oct 6. doi: 10.1038/nsmb.2680."
COMMENT     Sequence Label: pTALYM3
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    236..257
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(272..277,278..295,296..302)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    310..326
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     315..337
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     334..350
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     334..350
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             346..753
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     369..389
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        371..389
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(436..455)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(437..455)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(465..482)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(465..481)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(474..496)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cccttgctca ccatggagga ggcggtgccg gagccacctc cgcctccagt ggatcccgca
       61 acgcgatggg acgtgcgttc gccaatacgg cgattgactc ttctgatcaa ttccggcgcg
      121 tgcggcaatc cctttttcac tgcatccatg gcaggacgtc cgccgaggca ggccaaggcg
      181 acgaggtggt cgttggagac gaccggtgga aagcgggcag tgagcgcaac gcaattaatg
      241 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
      301 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
      361 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggaggacctc gagggggggc
      421 ccggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
      481 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
      541 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
      601 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cccgtctcca ggggggcacc
      661 cgtcagtgca ttgcgcgatg catgcactgc ctccattgcg gtcacgccgc cacgttttgc
      721 aatcttcaca agttggcctg tgtccaactg taac
//
