LOCUS       sequence-72293-e        5659 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of Etv5 S142D mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Znosko WA, Yu S, Thomas K, Molina GA, Li C, Tsang W, Dawid IB, Moon 
            AM, Tsang M
  TITLE     Overlapping functions of Pea3 ETS transcription factors in FGF 
            signaling during zebrafish development.
  JOURNAL   Dev Biol. 2010 Jun 1;342(1):11-25. Epub 2010 Mar 24.
  PUBMED    20346941
REFERENCE   2  (bases 1 to 5659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol.";
            date: "2010-06-1"; volume: "342(1)"; pages: "11-25. Epub 2010 Mar 
            24"
COMMENT     Sequence Label: Cs2+Etv5 S142D
FEATURES             Location/Qualifiers
     source          1..5659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    1708..1842
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1732..1751)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1786..1805
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1955..1975)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1955..1973)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1994..2010)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1994..2010)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2007..2029)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2018..2034
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2042..2048,2049..2066,2067..2072))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2087..2108
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2225..2242)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2396..2984)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2476..2495)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3155..3946,3947..4015))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3778..3797
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4016..4120)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4146..4601)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4283..4304)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4495..4514
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4675..5659
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaattca aggcctctcg agatggacgg
      121 attttatgac cagcaagttc catttatggt cccacctaat aagtcattgc aagtggagga
      181 accatataac agaccagtta atgacagaaa gagaaagtta gtggagactg aacttgctca
      241 ggacacagag gaacttttcc aggacctcag ccagctgcag gagatctgga ttgcagaagc
      301 tcaggtgccc gatgacgaac agtttgtccc agatttccag tcggagagct tgatgtttca
      361 tggcccaccc ctggccaaga tcaaacggga actgagtccc tccaaagagc tctccccctg
      421 tagccaggat agaagtccca tgccgtatgg agagaagtgc ctttacagct acagtgccta
      481 tgaacggaag cccagcactg ggttcaagcc attgactcct ccctcgacgc ccgtcgaccc
      541 atgtgtcccc agcaatgccc tggcgacgcg tcccctgcac gaacagactc caccacctgt
      601 gtccaacaca gcactggggc agcgtctcct tcatggacag cctacaatga ccaagagcac
      661 acccaaccag acgtgtgtcc ctcaccacag ccagagccca ccctttgtag tgccctgtcc
      721 atccctgaac cgtgaaactc acttcagtaa cgaacctagg tttcagcggc aactttcaga
      781 gccttgcctt ccattcccac cttctgatgc ccagggaaga cccagcttta tgccacaggt
      841 cgcctgcact tcaccaagag atggaagacc accgtaccat cgccaaatgt ctgagcccct
      901 ggttcctgta cctcctcagg gcttcaaaca ggagctcctc gacccccgct atgttgagca
      961 gggtgttccc aacatgggcc catcccaggc tgccttccac ccgatggcca tcaagcaaga
     1021 gccacgggac ttctgcttcg actctgaagt gcctaaatgt cagtcttcat ttgggagagc
     1081 agcaagcttc taccaaaacc atgagaactt ctcatttgac agagaacagc agttgtattt
     1141 tgatgacacc tgtgtggttc cagagagact tgaagggaag gtaaagcaag agccctctgt
     1201 gtatcgtgaa ggtccaccat atcagcggcg cggatctctc cagctgtggc agtttctggt
     1261 gacgctattg gatgatcctt ccaacggcca tttcattgcc tggactggac gtggaatgga
     1321 gttcaaactt attgagccag aggaggtggc tcgacgctgg ggcatccaga agaacagacc
     1381 cgccatgaac tatgacaagc tgagtcgctc actgcgctac tactatgaga agggaatcat
     1441 gcagaaggtt gccggcgaga ggtacgtcta caaatttgtg tgcgatcctg aagcgctctt
     1501 ctccatggcc ttcccagaca accagaggcc caatctcaaa gcagaccctg acggcctgcc
     1561 cactgtggat gatgacacac ttccattggc acattatgac gacgggtcct cgtatttagt
     1621 tgacggtggc gagcagtgtg tttctgggat gcctttccct gatggttacg tgtactgatc
     1681 tagaactata gtgagtcgta ttacgtagat ccagacatga taagatacat tgatgagttt
     1741 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     1801 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     1861 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaattcgc ggccgcggcg
     1921 ccaatgcatt gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct
     1981 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     2041 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     2101 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     2161 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     2221 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     2281 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     2341 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     2401 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     2461 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     2521 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     2581 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     2641 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     2701 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2761 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     2821 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     2881 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     2941 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     3001 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     3061 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     3121 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     3181 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     3241 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     3301 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     3361 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     3421 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     3481 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     3541 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     3601 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     3661 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     3721 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     3781 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     3841 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     3901 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     3961 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     4021 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     4081 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     4141 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     4201 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     4261 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     4321 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     4381 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     4441 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     4501 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     4561 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
     4621 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agtcgaccat
     4681 agccaattca atatggcgta tatggactca tgccaattca atatggtgga tctggacctg
     4741 tgccaattca atatggcgta tatggactcg tgccaattca atatggtgga tctggacccc
     4801 agccaattca atatggcgga cttggcacca tgccaattca atatggcgga cttggcactg
     4861 tgccaactgg ggaggggtct acttggcacg gtgccaagtt tgaggagggg tcttggccct
     4921 gtgccaagtc cgccatattg aattggcatg gtgccaataa tggcggccat attggctata
     4981 tgccaggatc aatatatagg caatatccaa tatggcccta tgccaatatg gctattggcc
     5041 aggttcaata ctatgtattg gccctatgcc atatagtatt ccatatatgg gttttcctat
     5101 tgacgtagat agcccctccc aatgggcggt cccatatacc atatatgggg cttcctaata
     5161 ccgcccatag ccactccccc attgacgtca atggtctcta tatatggtct ttcctattga
     5221 cgtcatatgg gcggtcctat tgacgtatat ggcgcctccc ccattgacgt caattacggt
     5281 aaatggcccg cctggctcaa tgcccattga cgtcaatagg accacccacc attgacgtca
     5341 atgggatggc tcattgccca ttcatatccg ttctcacgcc ccctattgac gtcaatgacg
     5401 gtaaatggcc cacttggcag tacatcaata tctattaata gtaacttggc aagtacatta
     5461 ctattggaag gacgccaggg tacattggca gtactcccat tgacgtcaat ggcggtaaat
     5521 ggcccgcgat ggctgccaag tacatcccca ttgacgtcaa tggggagggg caatgacgca
     5581 aatgggcgtt ccattgacgt aaatgggcgg taggcgtgcc taatgggagg tctatataag
     5641 caatgctcgt ttagggaac
//
