LOCUS       sequence-72320-e        1126 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV TALE synthesis backbone with NI half repeat; fused to nuclear 
            localization sequence linker and SID4X transcriptional repressor 
            effector domain. Synapsin promoter for neuronal expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Konermann S, Brigham MD, Trevino A, Hsu PD, Heidenreich M, Le Cong, 
            Platt RJ, Scott DA, Church GM, Zhang F
  TITLE     Optical control of mammalian endogenous transcription and epigenetic
            states.
  JOURNAL   Nature. 2013 Jul 23. doi: 10.1038/nature12466.
  PUBMED    23877069
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2013 Jul 23. doi: 10.1038/nature12466."
COMMENT     Sequence Label: 
            pAAV_hSyn_TALEBB(NI)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA
FEATURES             Location/Qualifiers
     source          1..1126
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(398..451)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(851..871)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt atcgataagc ttgatatcga
       61 attcttacac atgatcactt cccaccagct gcacaccgat gaagtattgc agccctccct
      121 ttcgatcccg gacaggttgg agatgaagca gattccagaa ctttttgcct gacttagtgt
      181 agtttattaa ctgcacagtg gtttccctct gatcgcggat agcatcgcga atcttctgaa
      241 cggtggcctg gtctgtctca ggcccttgca ggaacctggc gtttctcccc atgatttcct
      301 ccctggaata ttcggtgagc tccagaaaac cgtcgctggc aaaaatgatg gggtaatcag
      361 gcagtcttgg atctgttatt acgaaggact tctcgattgg gccaggattc tcctcgacgt
      421 caccgcatgt tagcagactt cctctgccct ctccactgcc ccatgggggc agcatagagg
      481 catagccatg ttcagcttct ctctcgcgcc tctccaggta atcggcagcc tccagcagca
      541 tttgtatgtt catcccgctg cctggcagca tgctggcgta tccgtgctcg gcttctcttt
      601 ctctccgttc caggtaatcg gctgcctcca gcagcatttg gatattcatt ccagacccag
      661 gcagcatgga agcatagcca tgttcggcct cccgttcgcg cctttccagg taatcagcag
      721 cctccagcag catctgaata ttcatgccgc ttccgggcag cattgaagca tatccgtgct
      781 cggcttcgcg ctctctccgt tccaggtagt cagcggcctc cagcagcatc tggatgttca
      841 tgctggcctc cacctttctc ttcttcttgg ggctgctagc tgccactcga tgtgatgtcc
      901 tctcgggaat ccttctgttg gtccgcttaa tcaatgcagg cgcgtgcggg agccccttct
      961 tcaccgcatc caaggcgggt cgtccgccga ggcaggccag cgctacaaga tgatcattag
     1021 tgagtgcggc cagcgcgggg tccggcctcg aaagctgggc cacgattgac tccagtgcgg
     1081 gtctgccccc gatattggat gcaatagcca ctacctgctc aggcgt
//
