LOCUS       sequence-72328-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Machida K, Thompson CM, Dierck K, Jablonowski K, Karkkainen S, Liu 
            B, Zhang H, Nash PD, Newman DK, Nollau P, Pawson T, Renkema GH, 
            Saksela K, Schiller MR, Shin DG, Mayer BJ
  TITLE     High-throughput phosphotyrosine profiling using SH2 domains.
  JOURNAL   Mol Cell. 2007 Jun 22;26(6):899-915.
  PUBMED    17588523
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2007-06-22"; volume: "26(6)"; pages: "899-915"
COMMENT     Sequence Label: pGEX Tec-SH2
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             410..454
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     primer_bind     complement(518..540)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(685..703)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        771..875
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agttctgttc caggggcccc tgggatccgg aaagaaatca aacaacttag atcaatatga
       61 atggtattgc agaaatatga atagaagcaa ggcagagcaa ctcctccgca gtgaagataa
      121 agaaggtggt tttatggtaa gggattccag tcaaccaggc ttgtacacag tctcccttta
      181 taccaagttt ggaggagaag gttcatcggg ttttaggcat tatcatataa aggaaacaac
      241 aacatctcca aagaagtatt acctagctga aaaacatgct tttggctcca ttcctgagat
      301 tattgaatat cataagcaca atgcagcagg acttgtcacc aggcttcggt acccagttag
      361 tgtgaaaggg aagaatggaa ttcatcgtga cgcaagcttg cttggtggcg gtctgaacga
      421 catcttcgag gctcagaaaa tcgaatggca cgaataagcg gccgcatcgt gactgactga
      481 cgatctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
      541 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
      601 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
      661 tgtataattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
      721 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      781 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      841 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
      901 cccttattcc cttttt
//
