LOCUS       sequence-72450-e         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the UBQ10 terminator as GreenGate module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGE009
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        8..26
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     8..25
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(696..715)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(697..715)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(725..747)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(941..960)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acgagctatt taggtgacac tatagaagtg aagcttggtc tcactgcatc tcgtctctgt
       61 tatgcttaag aagttcaatg tttcgtttca tgtaaaactt tggtggtttg tgttttgggg
      121 ccttgtataa tccctgatga ataagtgttc tactatgttt ccgttcctgt tatctctttc
      181 tttctaatga caagtcgaac ttcttcttta tcatcgcttc gtttttatta tctgtgcttc
      241 ttttgtttaa tacgcctgca aagtgactcg actctgttta gtgcagttct gcgaaacttg
      301 taaatagtcc aattgttggc ctctagtaat agatgtagcg aaagtgttga gctgttgggt
      361 tctaaggatg gcttgaacat gttaatcttt taggttctga gtatgatgaa cattcgttgt
      421 tgctaagaaa tgcctgtaat gtcccacaaa tgtagaaaat ggttcgtacc tttgtccaag
      481 cattgatatg tctgatgaga ggaaactgca agatactgag cttggtttaa cgaaggagag
      541 gcagtttctt ccttccaaag catttcattt gacaatgcct tgatcatctt aagtagagtt
      601 tctgttgtgg aaagtttgaa actttgaaga aacgactctc aagtaaattg atgatcacaa
      661 gtgaaagtgt atgttactat gagaccgaat tctcgcccta tagtgagtcg tattacctgg
      721 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
      781 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
      841 cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc
      901 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
      961 ca
//
