LOCUS       sequence-72508-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides linker:mCherry as GreenGate C-terminal tag module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGD003
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..739)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(307..326)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     576..594
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        complement(862..880)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(863..880)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ctatagggcg agaattcggt ctcagcagct acttgtacag ctcgtccatg ccgccggtgg
       61 agtggcggcc ctcggcgcgt tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg
      121 tgatgtccaa cttgatgttg acgttgtagg cgccgggcag ctgcacgggc ttcttggcct
      181 tgtaggtggt cttgacctca gcgtcgtagt ggccgccgtc cttcagcttc agcctctgct
      241 tgatctcgcc cttcagggcg ccgtcctcgg ggtacatccg ctcggaggag gcctcccagc
      301 ccatcgtctt cttctgcatt acggggccgt cggaggggaa gttggtgccg cgcagcttca
      361 ccttgtagat gaactcgccg tcctgcaggg aggagtcctg ggtcacggtc accacgccgc
      421 cgtcctcgaa gttcatcacg cgctcccact tgaagccctc ggggaaggac agcttcaagt
      481 agtcggggat gtcggcgggg tgcttcacgt aggccttgga gccgtacatg aactgagggg
      541 acaggatgtc ccaggcgaag ggcagggggc cacccttggt caccttcagc ttggcggtct
      601 gggtgccctc gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg tggccgttca
      661 cggagccctc catgtgcacc ttgaagcgca tgaactcctt gatgatggcc atgttatcct
      721 cctcgccctt gctcaccatg gctgcagctg cgatcgccga tccagagccg ctgccggaac
      781 cactccctga gcccgatcca gagccgcttc cggaaccact ccctgaagcg gccgctgcac
      841 ctgatgagac caagcttcac ttctatagtg tcacctaaat agctcgtgtg aaattgttat
      901 c
//
