LOCUS       sequence-72579-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant protein production .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Posner M, Kiss AJ, Skiba J, Drossman A, Dolinska MB, Hejtmancik JF, 
            Sergeev YV
  TITLE     Functional validation of hydrophobic adaptation to physiological 
            temperature in the small heat shock protein alphaA-crystallin.
  JOURNAL   PLoS One. 2012;7(3):e34438. doi: 10.1371/journal.pone.0034438. Epub 
            2012 Mar 29.
  PUBMED    22479631
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3):e34438. doi"; pages: " 
            10.1371/journal.pone.0034438. Epub 2012 Mar 29"
COMMENT     Sequence Label: Notothenia angustata cryaa
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             570..587
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(640..658)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      654..701
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(825..844)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatggaaat ttctatccag catccctggt
       61 tcagacgggc catgggctcc gtctaccccg ccagactctt tgaccagttc ttcggagagg
      121 gcatgtttga ctacgacttc ttcccctaca ccacctccag catcagcccc tactacagac
      181 agtccctgtt ccgaagcttc ttggattcct caaactccgg cacctctgag gtgaggtctg
      241 acagggataa gtacactttg tacctggatg tcaaacactt ctctcctgaa gagatcagtg
      301 tgaaggtgac cggggactac gtggagatcc agggcaagca tggagagaga caggatgacc
      361 acggttacat ctctcgtgag ttccaccgcc gctatcgcct cccctccagt gtggaccaat
      421 cagcaatcag ctgtagcctg tcagctgacg ggctgctgac cctgagcggg ccaaaggtca
      481 acggggggac cgaatcaggc cgcagcgagc gcagcatccc tgtcacccgc gacgagaaga
      541 ccaactctgc tgtgtcctcc tagctcgagc accaccacca ccaccactga gatccggctg
      601 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
      661 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
      721 ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
      781 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
      841 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
      901 tttagggttc cgatttagtg cttta
//
