LOCUS       sequence-72586-e         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Frank S, Skryabin BV, Greber B
  TITLE     A modified TALEN-based system for robust generation of knock-out 
            human pluripotent stem cell lines and disease models.
  JOURNAL   BMC Genomics. 2013 Nov 9;14(1):773.
  PUBMED    24206569
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2013-11-9"; volume: "14(1)"; pages: "773"
COMMENT     Sequence Label: pTAL7b
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..782)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQQKLEDLEGWPVTQFAARSLAVVLQRRDWENPGVTQL
                     NRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLQGGSCQSIPPSVHSFH
                     SCHSAAFSDLQQLSSVQSGLEWWTPQFPGDGEECFECSGTAPLLSDAHNGFVGCLRQSG
                     DHVLIFDGHSPECSGMLRQSHNMGVCETVPHQCFMVLSDGGPHLWFDLLLLLLAVAEGS
                     QIHLGCWTGGYPTSATTSATWFIVVILVVSGRHLPLFL"
     CDS             30..53
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     671..693
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     685..702
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     686..702
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(778..794)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(778..794)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(791..813)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    802..818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(826..832,833..850,851..856))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    871..892
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctaagaaaaa gaggaaggtg gcggccgctg actacaagga tgacgacgat aaaccaggtg
       61 gcggaggtag tggcggaggt ggggtacccg ccagtccagc agcccaggtg gatctgagaa
      121 ccctcggcta cagccagcag cagcaggaga agatcaaacc aaaggtgcgg tccaccgtcg
      181 ctcagcacca tgaagcactg gtggggcacg gtttcacaca cgcccatatt gtggctctgt
      241 ctcagcatcc cgctgcactc gggactgtgg ccgtcaaata tcaggacatg atcgccgctc
      301 tgcctgaggc aacccacgaa gccattgtgg gcgtcggaaa gcagtggagc ggtgccagag
      361 cactcgaagc actcctcacc gtcgccgggg aactgcgggg tccaccactc cagtccggac
      421 tggacactgg acagctgctg aagatcgcta aacgcggcgg agtgacagct gtggaagctg
      481 tgcacgcttg gaggaatgct ctgacaggag cccccctgga gacgggcgcc gctacagggc
      541 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
      601 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
      661 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc ggcgaattgg
      721 gtaacgggcc acccctcgag gtcctccagc ttttgttgcg cgcttggcgt aatcatggtc
      781 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
      841 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
      901 gcgctcactg cccgctttcc accggtcgtc tccaacgatc atctggtcgc tctcgcttgc
      961 ctcggtggac ggc
//
