LOCUS       sequence-72597-e        1009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Andersson CR, Tsinoremas NF, Shelton J, Lebedeva NV, Yarrow J, Min 
            H, Golden SS
  TITLE     Application of bioluminescence to the study of circadian rhythms in 
            cyanobacteria.
  JOURNAL   Methods Enzymol. 2000;305:527-42.
  PUBMED    10812624
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2000"; volume: "305"; pages: "527-42"
COMMENT     Sequence Label: pAM1573
FEATURES             Location/Qualifiers
     source          1..1009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(94..121)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(213..299)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     639..658
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(877..981)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgggtaaccg atatcgtcga caggcctcta gacccgggct cgagctagca agcttggccg
       61 gatccggccg gatccggagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc
      121 tgcttaattt gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt
      181 tgcttcgcaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc
      241 gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga
      301 tgcctggcag ttccctactc tcgcatgggg agaccccaca ctaccatcgg cgctacggcg
      361 tttcacttct gagttcggca tggggtcagg tgggaccacc gcgctactgc cgccaggcaa
      421 attctgtttt atcagccgtt accccaccta ctagctaatc ccatctgggc acatccgatg
      481 gcaagaggcc cgaaggtccc cctctttggt cttgcgacgt tatgcggtat tagctaccgt
      541 ttccagtagt tatccccctc catcaggcag tttcccagac attactcacc cgtccgccac
      601 tcgtcagcaa agaagcaagc ttagatccgt cacacgggat aataccgcgc cacatagcag
      661 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
      721 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
      781 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
      841 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
      901 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
      961 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgac
//
