LOCUS       sequence-72681-e        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, 
            Bleomycin/Gancyclovir selectable.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Kim HS, Li Z, Boothroyd C, Cross GA
  TITLE     Strategies to construct null and conditional null Trypanosoma brucei
            mutants using Cre-recombinase and loxP.
  JOURNAL   Mol Biochem Parasitol. 2013 Aug 13;191(1):16-19. doi: 
            10.1016/j.molbiopara.2013.08.001.
  PUBMED    23954366
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molbiopara.2013.08"; journalName: "Mol Biochem 
            Parasitol"; date: "2013-08-13- 13"; volume: "191"; issue: "1"; 
            pages: "16-19"
COMMENT     Sequence Label: pHJ42
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    291..324
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cggcgaagtc gtcctccacg aagtcccggg agaacccgag ccggtcggtc cagaactcga
       61 ccgctccggc gacgtcgcgc gcggtgagca ccggaacggc actggtcaac ttggccatgg
      121 tgagatctgt aatgtcagtg cgagcgatca agaatgtaac acaaaggaat accaagtttg
      181 tgaattttat tttttggtgt aattgaagtc ttcaagttga aaaaaatggg gaaaaggaaa
      241 aaaaactcga aggcgcgtgc agcggccggc cgacgagaac gtgtaccggt ataacttcgt
      301 ataatgtatg ctatacgaag ttataagctt cgttgcagtt gagtttctgt tttcgggctt
      361 gttggtatac ttgtttgatt aatgtggctg atagctaagc tatcgcaaat atttgtacaa
      421 gacatgaggg ggcaaaaaaa taagccaagt gtatttgtta ggcttccgtg cctgttagag
      481 aaatatgtaa ggaaacgcgt gcgctacaca gcttgagcac cctcgagcat cttcattacc
      541 tactgctaat gacgaacggg aaatgccccg tccgcgtcta gtattgctcc tcctcgtcga
      601 actcgccctc ctcctcaatc gtggcatcct ggtactgctg gtactcagac acgagatcgt
      661 tcatgttgga ctctgcctcc gtgaattcca tctcgtccat accctcgcca gtgtaccagt
      721 gcaagaacgc cttgcgacgg aacatgaggg tgaactgctc tcccacacgg cggaacatct
      781 cctggatgca ggtgttgttg ccaatgaagg tgacagccat cttgagtccc ttgggtggga
      841 tatcgcaaac agaggacttg atgttgttcg ggatctccac tcaatgaagt aggacgagtt
      901 cttgttctgc acgttcagca tctgctcatc aacctccttc gtcgacatgc ggccgcggaa
      961 gagtgcagac gctgtcaggt agcggccgtg acgaggatct gcagcttgca tcatgttttt
     1021 cgcatcgaac atctgctgcg ttagctcggg
//
