LOCUS       sequence-72683-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial nuclease-null SP Cas9 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: DS-SPcasN-
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctaacaccac gtcgtcccta tctgctgccc taggtctatg agtggttgct ggataacttt
       61 acgggcatgc ataaggctcg tatgatatat tcagggagac cacaacggtt tccctctaca
      121 aataattttg tttaactttt actagaggag gaggcaaaaa tggacaagaa gtactccatt
      181 gggctcgcta tcggcacaaa cagcgtcggc tgggccgtca ttacggacga gtacaaggtg
      241 ccgagcaaaa aattcaaagt tctgggcaat accgatcgcc acagcataaa gaagaacctc
      301 attggcgccc tcctgttcga ctccggggag acggccgaag ccacgcggct caaaagaaca
      361 gcacggcgca gatatacccg cagaaagaat cggatctgct acctgcagga gatctttagt
      421 aatgagatgg ctaaggtgga tgactctttc ttccataggc tggaggagtc ctttttggtg
      481 gaggaggata aaaagcacga gcgccaccca atctttggca atatcgtgga cgaggtggcg
      541 taccatgaaa agtacccaac catatatcat ctgaggaaga agcttgtaga cagtactgat
      601 aaggctgact tgcggttgat ctatctcgcg ctggcgcata tgatcaaatt tcggggacac
      661 ttcctcatcg agggggacct gaacccagac aacagcgatg tcgacaaact ctttatccaa
      721 ctggttcaga cttacaatca gcttttcgaa gagaacccga tcaacgcatc cggagttgac
      781 gccaaagcaa tcctgagcgc ta
//
