LOCUS       sequence-7270-ex       10455 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10455)
  TITLE     Trono Lab Constitutive Lentiviral Plasmids
REFERENCE   2  (bases 1 to 10455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pWPXLd
FEATURES             Location/Qualifiers
     source          1..10455
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2120..3297
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2351..3288
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3245..3265
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3345..3462
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      3538..3547
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3544..3546,3547..3549,3550..4260)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3544..3546,3547..3549,3550..4260)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3589..3610)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3850..3869)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4197..4218
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4340..4928
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4393..4413)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4811..4822)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5017..5250
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5275..5293)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5276..5293)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5583..5602)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5816..5835)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5935..5957
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5995..6013)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6081..6185
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6186..6254,6255..7046)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6404..6423)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7217..7805
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7706..7725
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7959..7976
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8046..8066)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8051..8380
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8231..8366
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9514..9579
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9709..9729
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10154..10288
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10191..10210)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10245..10264
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatgtcgac gataagcttt
     2041 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     2101 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     2161 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     2221 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     2281 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     2341 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     2401 cccttgcgtg ccttgaatta cttccactgg ctgcagtacg tgattcttga tcccgagctt
     2461 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     2521 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     2581 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     2641 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
     2701 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
     2761 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     2821 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     2881 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     2941 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     3001 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     3061 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     3121 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     3181 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     3241 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga
     3301 atttcgacat ttaaatttaa ttaatctcga cggtatcggt taacttttaa aagaaaaggg
     3361 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     3421 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttccgatcac gagactagcc
     3481 tcgaggttta aactacggga tccaggccta agcttacgcg tcctagcgct accggtcgcc
     3541 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     3601 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     3661 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     3721 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     3781 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     3841 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     3901 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     3961 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     4021 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     4081 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     4141 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     4201 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     4261 tccggactca gatctcgata gctagtagct agctagctag tcgagctcaa cttcgaattc
     4321 ccgggactag tcatatgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     4381 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     4441 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     4501 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     4561 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     4621 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     4681 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     4741 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     4801 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     4861 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     4921 ctccccgcat cggtacgtat ggccaggtac ctttaagacc aatgacttac aaggcagctg
     4981 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     5041 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     5101 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     5161 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     5221 ccttttagtc agtgtggaaa atctctagca gcatctagaa ttaattccgt gtattctata
     5281 gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc
     5341 taacgacaat atgtacaagc ctaattgtgt agcatctggc ttactgaagc agaccctatc
     5401 atctctctcg taaactgccg tcagagtcgg tttggttgga cgaaccttct gagtttctgg
     5461 taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc agcagggtca tcgctagcca
     5521 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
     5581 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
     5641 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
     5701 ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg
     5761 ctgcttccta atgcaggagt cgcataaggg agagcgtcga atggtgcact ctcagtacaa
     5821 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     5881 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     5941 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     6001 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     6061 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     6121 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     6181 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     6241 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     6301 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     6361 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     6421 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     6481 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     6541 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     6601 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     6661 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     6721 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     6781 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     6841 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     6901 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     6961 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     7021 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     7081 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     7141 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7201 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     7261 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     7321 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     7381 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7441 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7501 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7561 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg
     7621 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7681 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7741 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7801 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7861 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     7921 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     7981 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     8041 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     8101 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     8161 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     8221 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     8281 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     8341 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg
     8401 cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa
     8461 aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc
     8521 aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca
     8581 tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa
     8641 gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt
     8701 accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc
     8761 cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg
     8821 cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg
     8881 tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct
     8941 ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat
     9001 gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc
     9061 gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg
     9121 ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc
     9181 ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg
     9241 cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct
     9301 ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg atgagcgatg
     9361 ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc ggcaactgga
     9421 tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg
     9481 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa
     9541 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat
     9601 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg
     9661 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag
     9721 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct
     9781 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca
     9841 ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt
     9901 tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat
     9961 aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa
    10021 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
    10081 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
    10141 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    10201 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    10261 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
    10321 caaacttccc accccatacc ctattaccac tgccaattac ctagtggttt catttactct
    10381 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
    10441 ctacaaactt agtag
//
