LOCUS       sequence-72701-e         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLAG-intergenic region of TUB-HYG (modified from PMID 16269191).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Kim HS, Li Z, Boothroyd C, Cross GA
  TITLE     Strategies to construct null and conditional null Trypanosoma brucei
            mutants using Cre-recombinase and loxP.
  JOURNAL   Mol Biochem Parasitol. 2013 Aug 13;191(1):16-19. doi: 
            10.1016/j.molbiopara.2013.08.001.
  PUBMED    23954366
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molbiopara.2013.08"; journalName: "Mol Biochem 
            Parasitol"; date: "2013-08-13- 13"; volume: "191"; issue: "1"; 
            pages: "16-19"
COMMENT     Sequence Label: pDS69
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      180..189
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      184..193
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(223..242)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(224..242)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(249..266)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(249..265)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(258..280)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      406..861
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(493..512)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     703..724
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
       61 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
      121 cgacgcccca gcactcgtcc gagggcaaag gaatcttcta gaaaaatcga attcccgcgg
      181 ccgccatggc ggccgggagc atgcgacgtc gggcccaatt cgccctatag tgagtcgtat
      241 tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      301 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      361 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg
      421 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
      481 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
      541 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
      601 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
      661 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
      721 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
      781 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
      841 aaatattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat
      901 ttcacaccgc atcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
      961 ttttctaaat acattcaaat atgtatccgc tcatga
//
