LOCUS       sequence-72723-e         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian NM-VP64 nuclease-null Cas9 activator expression, human 
            optimized.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: M-NMn-VP64
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(37..186)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(211..231)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      648..657
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accggtaggg atcgaaccct ttcatctaga gttaatcagc atgtccaggt cgaaatcatc
       61 aagggcgtca ctgccgagca tgtcgaggtc aaagtcatca agggcatccg aaccaagcat
      121 gtcaaggtca aaatcatcga gggcgtcact tcccagcata tccagatcaa aatcgtccaa
      181 tgcgtcagcc cgtccggaac cgctggcctc caccttcctc ttcttcttgg ggtcagccct
      241 gctgcgcaca ggagggcgct tcttcaggcg gcaggggcgg atctccttgc ccagctcgtc
      301 gatctggtac ttctggaagc tcagggcggt cttcacgccg atgccctcca ggatgccgtt
      361 cttgccgatc ttgtggtcca ggtcgtggat gcggatgttg atgttgccgg tgccgcggtg
      421 gcagctggcg aagtagccga acatgcgggc cttcttggtg atcacctcca ccaggtcgtt
      481 ggggtgcagg ctgaacttga agttgaagct gtcgtcgatc agctgccagt cctcctcgtc
      541 cttgccctgc accacggcgc ggtcgggcag gatgcccttg gccacctgcc agctgtagat
      601 gggcaccagg tagtacttgt cgcccttctc gaacacgtcc acgcgcacca tggtggcgtt
      661 gtcggcgatg ccgttgtggt tgcgcaccca cacgccggtc ttctgcacct gctccacgcg
      721 cacggccttc acctgctggg tgcggttgcc ggccttgtcg tacttgtaga agggctcggc
      781 gaaggccttg gcggggtcgt ccttgtgggc ctccaggcgg gccttcaggg cctcgtacag
      841 cttgggctcg cgctcgcggt tcaccatctt ctccaggtcc ttcagcttca gctgggtcag
      901 gggcacgcgc agcacgctca cgccctcgtc caggcgcttg gcgctcttca cggtctccat
      961 gtgaccctga ccgctcatct tgcggttggg
//
