LOCUS       sequence-72730-e        1063 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, 
            Puromycin//Gancyclovir selectable.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Kim HS, Li Z, Boothroyd C, Cross GA
  TITLE     Strategies to construct null and conditional null Trypanosoma brucei
            mutants using Cre-recombinase and loxP.
  JOURNAL   Mol Biochem Parasitol. 2013 Aug 13;191(1):16-19. doi: 
            10.1016/j.molbiopara.2013.08.001.
  PUBMED    23954366
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molbiopara.2013.08"; journalName: "Mol Biochem 
            Parasitol"; date: "2013-08-13- 13"; volume: "191"; issue: "1"; 
            pages: "16-19"
COMMENT     Sequence Label: pHJ18
FEATURES             Location/Qualifiers
     source          1..1063
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    158..191
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gtcagtgcga gcgatcaaga atgtaacaca aaggaatacc aagtttgtga attttatttt
       61 ttggtgtaat tgaagtcttc aagttgaaaa aaatggggaa aaggaaaaaa aactcgaagg
      121 cgcgtgcagc ggccggccga cgagaacgtg taccggtata acttcgtata atgtatgcta
      181 tacgaagtta taagcttcgt tgcagttgag tttctgtttt cgggcttgtt ggtatacttg
      241 tttgattaat gtggctgata gctaagctat cgcaaatatt tgtacaagac atgagggggc
      301 aaaaaaataa gccaagtgta tttgttaggc ttccgtgcct gttagagaaa tatgtaagga
      361 aacgcgtgcg ctacacagct tgagcaccct cgagcatctt cattacctac tgctaatgac
      421 gaacgggaaa tgccccgtcc gcgtctagta ttgctcctcc tcgtcgaact cgccctcctc
      481 ctcaatcgtg gcatcctggt actgctggta ctcagacacg agatcgttca tgttggactc
      541 tgcctccgtg aattccatct cgtccatacc ctcgccagtg taccagtgca agaacgcctt
      601 gcgacggaac atgagggtga actgctctcc cacacggcgg aacatctcct ggatgcaggt
      661 gttgttgcca atgaaggtga cagccatctt gagtcccttg ggtgggatat cgcaaacaga
      721 ggacttgatg ttgttcggga tctccactca atgaagtagg acgagttctt gttctgcacg
      781 ttcagcatct gctcatcaac ctccttcgtc gacatgcggc cgcggaagag tgcagacgct
      841 gtcaggtagc ggccgtgacg aggatctgca gcttgcatca tgtttttcgc atcgaacatc
      901 tgctgcgtta gctcgggcac ggagagaccg cggtactgct gcgagccgcg gctggtcagc
      961 ggggcgaagc ccatcatgaa gaagtgcaga cgcgggaatg ggacaaggtt cacagccaac
     1021 ttacggaggt cagagttcaa ctgaccaggg aagcgcaggc agc
//
