LOCUS       sequence-72733-e        1092 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, 
            Puromycin//Gancyclovir selectable.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Kim HS, Li Z, Boothroyd C, Cross GA
  TITLE     Strategies to construct null and conditional null Trypanosoma brucei
            mutants using Cre-recombinase and loxP.
  JOURNAL   Mol Biochem Parasitol. 2013 Aug 13;191(1):16-19. doi: 
            10.1016/j.molbiopara.2013.08.001.
  PUBMED    23954366
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molbiopara.2013.08"; journalName: "Mol Biochem 
            Parasitol"; date: "2013-08-13- 13"; volume: "191"; issue: "1"; 
            pages: "16-19"
COMMENT     Sequence Label: pHJ18
FEATURES             Location/Qualifiers
     source          1..1092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    222..255
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatctgg ctagcttgca
       61 tgcctgcaag ggggctcgaa tccccccatt ttcttctttt acatcatccc cccttcctaa
      121 ccaacctgca ggcgcacctc cctgctgtgc catcagatta ctccgggaac ctttccacgg
      181 aaaagacacg agacaaggga aaagccaaat gggcaggatc cataacttcg tataatgtat
      241 gctatacgaa gttatgaatt ctcagttagc ctcccccatc tcccgggcaa acgtgcgcgc
      301 caggtcgcat atcgtcggta tggagccggg ggtggtgacg tgggtctgga ccatcccgga
      361 ggtaagttgc agcagggcgt cccggcagcc ggcgggcgat tggtcgtaat ccaggataaa
      421 gacgtgcatg gaacggaggc gtttggccaa gacgtccaag gcccaggcaa acacgttata
      481 caggtcgccg ttgggggcca gcaactcggg ggcccgaaac agggtaaata acgtgtcccc
      541 gatatggggt cgtgggcccg cgttgctctg gggctcggca ccctggggcg gcacggccgt
      601 ccccgaaagc tgtccccagt cctcccgcca cgacccgccg cactgcagat accgcaccgt
      661 attggcaagt agcccgtaaa cgcggcgaat cgcagccagc atagccaggt ccagccgctc
      721 gccggggcgc tggcgtttgg ccaggcggtc gatgtgtctg tcctccggaa gggccccaag
      781 cacgatgttg gtgccgggca aggtcggcgg gatgagggcc acgaacgcca gcacggcctg
      841 gggggtcatg ctgcccataa ggtaccgcgc ggccgggtag cacagcatga cggcgatggg
      901 atggcggtcg aggaggaggg tgagggccgg gggcggggca tgtgagctcc cagcctcccc
      961 cccgatatga ggagccagaa cggcgtcggt cacggcataa ggcatgccca ttgttatctg
     1021 ggcgcttgtc attaccaccg ccgcgtcccc ggccgatatc tcaccctggt cgaggcggtg
     1081 ttgtgtggtg ta
//
