LOCUS       sequence-72828-e         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For inserting selection gene between AttR3 and AttL4 sites for use 
            in Gateway recombination cloning system (corresponds to a module 3 
            plasmid).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, 
            Dankort D
  TITLE     A modular lentiviral and retroviral construction system to rapidly 
            generate vectors for gene expression and gene knockdown in vitro and
            in vivo.
  JOURNAL   PLoS One. 2013 Oct 11;8(10):e76279. doi: 
            10.1371/journal.pone.0076279.
  PUBMED    24146852
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-11"; volume: "8(10):e76279. doi"; pages: " 
            10.1371/journal.pone.0076279"
COMMENT     Sequence Label: pBEG R3-L4   #BGV194
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(24..147)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(224..319)
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(348..367)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(349..367)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(374..391)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(374..390)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(383..405)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cgccactagc agatctgccg gcccatagtg actggatatg ttgtgtttta cagtattatg
       61 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
      121 ctcgttcaac tttattatac atagttggct agcgcggccg cacgcgtgct gaggagacat
      181 ctagactttc cctcagcgtc gacgatatcg gcgcgccccc gggcaacttt tctatacaaa
      241 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
      301 caaaataaaa tcattattta ctagtgttaa cttcgatccc caattcgccc tatagtgagt
      361 cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
      421 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
      481 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatga gcttgcgccg
      541 tcccgtcaag tcagcgtaat gctctgccag tgttacaacc aattaaccaa ttctgattag
      601 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
      661 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
      721 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
      781 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
      841 tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca
      901 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgat
//
