LOCUS       sequence-72832-e        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Bonnet J, Subsoontorn P, Endy D
  TITLE     Rewritable digital data storage in live cells via engineered control
            of recombination directionality.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 
            21.
  PUBMED    22615351
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-06-5"; volume: "109(23)"; pages: 
            "8884-9. Epub 2012 May 21"
COMMENT     Sequence Label: Phi80 JQ929581
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(14..60)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(86..796)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELY"
     protein_bind    838..885
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
ORIGIN
        1 cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg
       61 atgcctggct ctagtatcat catctgtaca gttcatccat accatgcgtg atgcccgctg
      121 cggttacgaa ctccagcaga accatatgat cgcgtttctc gttcggatct ttagacagaa
      181 cgctttgcgt gctcagatag tgattgtctg gcagcagaac aggaccatca ccgattggag
      241 tgttttgctg gtagtgatca gccagctgca cgctgccatc ctccacgttg tggcgaattt
      301 taaaattcgc tttaatgcca tttttttgtt tatcggcggt gatgtaaaca ttgtggctgt
      361 taaaattgta ttccagctta tggcccagga tattgccgtc ttctttaaag tcaatgcctt
      421 tcagctcaat gcggtttacc agggtatcgc cttcaaattt cacttccgca cgcgttttgt
      481 acgtgccgtc atccttaaag gaaatcgtgc gttcctgcac atagccttcc ggcatggcgg
      541 acttgaagaa gtcatgctgc ttcatatggt ccggataacg agcaaagcac tgaacaccat
      601 aagtcagcgt cgttaccaga gtcggccaag gtaccggcag tttaccagta gtacagatga
      661 acttcagcgt cagtttacca ttagttgcgt caccttcacc ctcgccacgc acggaaaact
      721 tatgaccgtt gacatcacca tccagttcca ccagaatagg gacgacacca gtgaacagct
      781 cttcgccttt acgcatatgt atatctcctt cttaaaagat cttttctcta gtatcgtggt
      841 ttgtctggtc aaccaccgcg gtctcagtgg tgtacggtac aaacccaata gcgaggaggt
      901 aaattgtaag ctttagggaa acgacattta ctgacgcgac tttctcaaag ctagggtgtt
      961 ggcctgaact gtacgcttgg agttgtagga cagacataaa agtatcagat acgcacacta
     1021 accatctgct agcattatac ctaggactga
//
