LOCUS       sequence-72846-e         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Supplies the selection marker (neomycin resistance) and IRES with 
            flanked by AttR2 and AttL3 sites for Gateway cloning recombination 
            reaction. (This corresponds to a module 3 plasmid)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, 
            Dankort D
  TITLE     A modular lentiviral and retroviral construction system to rapidly 
            generate vectors for gene expression and gene knockdown in vitro and
            in vivo.
  JOURNAL   PLoS One. 2013 Oct 11;8(10):e76279. doi: 
            10.1371/journal.pone.0076279.
  PUBMED    24146852
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-11"; volume: "8(10):e76279. doi"; pages: " 
            10.1371/journal.pone.0076279"
COMMENT     Sequence Label: pBEG R2-iNeo-L3   #BGV207
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    44..139
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(287..306)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     897..916
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 cgactcacta tagggcgaat tggggatcga agttaacact agtaaataat gattttattt
       61 tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca
      121 actttgtata ataaagttgc ccgggggcgc gccgatatct cgagacgcgt gatcctcaga
      181 agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt
      241 aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag
      301 ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag
      361 aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga
      421 gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc
      481 cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg
      541 ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat
      601 gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg
      661 acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga
      721 caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg
      781 cctcgtcctg cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc
      841 gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc
      901 agtcatagcc gaatagcctc tccacccaag cggccgga
//
