LOCUS       sequence-72988-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of UNC-60B (a C. elegans ADF/cofilin).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Ono S, Benian GM
  TITLE     Two Caenorhabditis elegans actin depolymerizing factor/cofilin 
            proteins, encoded by the unc-60 gene, differentially regulate actin 
            filament dynamics.
  JOURNAL   J Biol Chem. 1998 Feb 6;273(6):3778-83.
  PUBMED    9452511
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1998-02-6"; volume: "273(6)"; pages: "3778-83"
COMMENT     Sequence Label: pET-UNC60B
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(531..549)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      545..592
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 actttaagaa ggagatatac catggcttcc ggagtcaaag ttgatccatc ctgcaagaac
       61 gcctacgatc ttctccacaa caagcatcag cacagctaca tcatcttcaa gattgacaaa
      121 aatgacaccg ccatcgtcgt cgagaaggtc ggagagaaga atgctccgta cgccgagttc
      181 gttgaggaga tgaagaagct tgttgaggac ggaaaggagt gcagatatgc tgccgtcgac
      241 gtcgaggtta ctgtccaaag acagggagcc gagggaacca gcacactcaa taaggtcatc
      301 ttcgttcaat actgtccaga caacgcccca gtccgcagac gtatgctcta cgcctcctcg
      361 gtccgtgctc tcaaggcgtc cctcggactc gagtccctct tccaagtaca ggcctccgag
      421 atgtcggacc tcgacgagaa gagcgtgaag agcgacctga tgtccaacca aagaatctaa
      481 ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
      541 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
      601 aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag
      661 tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc
      721 cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt
      781 gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa
      841 ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt
      901 tagatttcat acacgg
//
