LOCUS       sequence-72993-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contributes the coding sequence for the mKate2 fluorescent protein 
            as the 3'-module during MultiSite Gateway-cloning of chimeric cDNAs 
            encoding three-part fusion proteins..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Buj R, Iglesias N, Planas AM, Santalucia T
  TITLE     A plasmid toolkit for cloning chimeric cDNAs encoding customized 
            fusion proteins into any Gateway destination expression vector.
  JOURNAL   BMC Mol Biol. 2013 Aug 20;14(1):18. doi: 10.1186/1471-2199-14-18.
  PUBMED    23957834
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Mol 
            Biol."; date: "2013-08-20"; volume: "14(1):18. doi"; pages: " 
            10.1186/1471-2199-14-18"
COMMENT     Sequence Label: pDONR P2R-P3-mKate2
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(54..177)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             180..875
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
ORIGIN
        1 cagctctaga gctcgaattc tacaggtcac taataccatc taagtagttg gttcatagtg
       61 actgcatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
      121 atatattgat atttatatca ttttacgttt ctcgttcaac tttcttgtac aaagtggaca
      181 tggtgagcga gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtga
      241 acaaccacca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga
      301 ccatgagaat caaggcggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta
      361 ccagcttcat gtacggcagc aaaaccttca tcaaccacac ccagggcatc cccgacttct
      421 ttaagcagtc cttccccgag ggcttcacat gggagagagt caccacatac gaagacgggg
      481 gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca
      541 agatcagagg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa acactcggct
      601 gggaggcctc caccgagacc ctgtaccccg ctgacggcgg cctggaaggc agagccgaca
      661 tggccctgaa gctcgtgggc gggggccacc tgatctgcaa cttgaagacc acatacagat
      721 ccaagaaacc cgctaagaac ctcaagatgc ccggcgtcta ctatgtggac agaagactgg
      781 aaagaatcaa ggaggccgac aaagagacct acgtcgagca gcacgaggtg gctgtggcca
      841 gatactgcga cctccctagc aaactggggc acagaggtgg aggaggttcc ggactcagat
      901 ctcgagctca agcttcgaat tctgcagtcg acggtaccgc ggg
//
