LOCUS       sequence-73006-e        1133 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA-Sumo1 WT in mammalian cells .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Bekes M, Prudden J, Srikumar T, Raught B, Boddy MN, Salvesen GS
  TITLE     The dynamics and mechanism of SUMO chain deconjugation by 
            SUMO-specific proteases.
  JOURNAL   J Biol Chem. 2011 Mar 25;286(12):10238-47. Epub 2011 Jan 19.
  PUBMED    21247896
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2011-03-25"; volume: "286(12)"; pages: "10238-47. 
            Epub 2011 Jan 19"
COMMENT     Sequence Label: pcDNA3 HA-Sumo1 WT
FEATURES             Location/Qualifiers
     source          1..1133
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             105..131
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(453..471)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(454..471)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(491..508)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    497..721
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      767..1125
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(854..873)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1064..1085
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttggtaccat ggcctaccca tacgacgtac
      121 cagactacgc acattctgac caggaggcaa aaccttcaac tgaggacttg ggggataaga
      181 aggaaggtga atatattaaa ctcaaagtca ttggacagga tagcagtgag attcacttca
      241 aagtgaaaat gacaacacat ctcaagaaac tcaaagaatc atactgtcaa agacagggtg
      301 ttccaatgaa ttcactcagg tttctctttg agggtcagag aattgctgat aatcatactc
      361 caaaagaact gggaatggag gaagaagatg tgattgaagt ttatcaggaa caaacggggg
      421 gttagctcga gcatgcatct agagggccct attctatagt gtcacctaaa tgctagagct
      481 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
      541 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
      601 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
      661 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
      721 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
      781 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
      841 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
      901 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
      961 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     1021 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     1081 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gat
//
