LOCUS       sequence-73050-e         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Ataxin1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Kim E, Lu HC, Zoghbi HY, Song JJ
  TITLE     Structural basis of protein complex formation and reconfiguration by
            polyglutamine disease protein Ataxin-1 and Capicua.
  JOURNAL   Genes Dev. 2013 Mar 15;27(6):590-5. doi: 10.1101/gad.212068.112.
  PUBMED    23512657
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.212068"; journalName: "Genes Dev"; date: "2013-03-15- 
            15"; volume: "27"; issue: "6"; pages: "590-5"
COMMENT     Sequence Label: Flag-ATXN1
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             213..236
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tggtaccgag ctcggatcca ctagtaacgg ccgccagtgt
      121 gctggaattc cgtgactttc cgtttatcag actaaaatca gagccatcca gacagtgaaa
      181 cagtcaccgt ggagggggga cggcgaaaaa tggactacaa agacgacgac gacaaaaaat
      241 ccaaccaaga gcggagcaac gaatgcctgc ctcccaagaa gcgcgagatc cccgccacca
      301 gccggtcctc cgaggagaag gcccctaccc tgcccagcga caaccaccgg gtggagggca
      361 cagcatggct cccgggcaac cctggtggcc ggggccacgg gggcgggagg catgggccgg
      421 cagggacctc ggtggagctt ggtttacaac agggaatagg tttacacaaa gcattgtcca
      481 cagggctgga ctactccccg cccagcgctc ccaggtctgt ccccgtggcc accacgctgc
      541 ctgccgcgta cgccaccccg cagccaggga ccccggtgtc ccccgtacag tacgctcacc
      601 tgccgcacac cttccagttc attgggtcct cccaatacag tggaacctat gccagcttca
      661 tcccatcaca gctgatcccc ccaaccgcca accccgtcac cagtgcagtg gcctcggccg
      721 caggggccac cactccatcc cagcgctccc agctggaggc ctattccact ctgctggcca
      781 acatgggcag tctgagccag acgccgggac acaaggctga gcagcagcac ctcagcaggg
      841 ctccggggct tatcaccccg gggtcccccc caccagccca gcagaaccag tacgtccaca
      901 tttccagttc tccg
//
