LOCUS       sequence-73053-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses L588D mutated Ataxin1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Kim E, Lu HC, Zoghbi HY, Song JJ
  TITLE     Structural basis of protein complex formation and reconfiguration by
            polyglutamine disease protein Ataxin-1 and Capicua.
  JOURNAL   Genes Dev. 2013 Mar 15;27(6):590-5. doi: 10.1101/gad.212068.112.
  PUBMED    23512657
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.212068"; journalName: "Genes Dev"; date: "2013-03-15- 
            15"; volume: "27"; issue: "6"; pages: "590-5"
COMMENT     Sequence Label: Flag-ATXN1_L588D
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             195..218
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
       61 cttggtaccg agctcggatc cactagtaac ggccgccagt gtgctggaat tccgtgactt
      121 tccgtttatc agactaaaat cagagccatc cagacagtga aacagtcacc gtggaggggg
      181 gacggcgaaa aatggactac aaagacgacg acgacaaaaa atccaaccaa gagcggagca
      241 acgaatgcct gcctcccaag aagcgcgaga tccccgccac cagccggtcc tccgaggaga
      301 aggcccctac cctgcccagc gacaaccacc gggtggaggg cacagcatgg ctcccgggca
      361 accctggtgg ccggggccac gggggcggga ggcatgggcc ggcagggacc tcggtggagc
      421 ttggtttaca acagggaata ggtttacaca aagcattgtc cacagggctg gactactccc
      481 cgcccagcgc tcccaggtct gtccccgtgg ccaccacgct gcctgccgcg tacgccaccc
      541 cgcagccagg gaccccggtg tcccccgtac agtacgctca cctgccgcac accttccagt
      601 tcattgggtc ctcccaatac agtggaacct atgccagctt catcccatca cagctgatcc
      661 ccccaaccgc caaccccgtc accagtgcag tggcctcggc cgcaggggcc accactccat
      721 cccagcgctc ccagctggag gcctattcca ctctgctggc caacatgggc agtctgagcc
      781 agacgccggg gacacaaggc tgagcagcag cacctcagca gggctcc
//
