LOCUS       sequence-73097-e        1075 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Pastrana DV, Tolstov YL, Becker JC, Moore PS, Chang Y, Buck CB
  TITLE     Quantitation of human seroresponsiveness to Merkel cell 
            polyomavirus.
  JOURNAL   PLoS Pathog. 2009 Sep . 5(9):e1000578.
  PUBMED    19750217
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog. 2009 Sep . 5(9):e1000578."
COMMENT     Sequence Label: pGwf
FEATURES             Location/Qualifiers
     source          1..1075
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(133..257)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    319..907
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(372..392)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(790..801)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
       61 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
      121 ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
      181 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct
      241 ttcttgtaca aagtggttcg atctagaatg gctagtggat cccccgggct gcaggaattc
      301 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
      361 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
      421 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
      481 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
      541 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
      601 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
      661 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
      721 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
      781 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
      841 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
      901 tccccgcatc gataccgtcg gcccactgct ccctaaacct gagctagcat tatccctaat
      961 acctgccacc ccactcttaa tcagtggtgg aagaacggtc tcagaactgt ttgtttcaat
     1021 tggccattta agtttagtag taaaagactg gttaatgata acaatgcatc gtaaa
//
