LOCUS       sequence-73098-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Frank S, Skryabin BV, Greber B
  TITLE     A modified TALEN-based system for robust generation of knock-out 
            human pluripotent stem cell lines and disease models.
  JOURNAL   BMC Genomics. 2013 Nov 9;14(1):773.
  PUBMED    24206569
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2013-11-9"; volume: "14(1)"; pages: "773"
COMMENT     Sequence Label: pTAL7a
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(80..97)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(80..96)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(89..111)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(729..752)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(762..782)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      785..794
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(798..817)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(799..817)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(884..906)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 tgaccatgat tacgccaagc gcgcaacaaa agctggagga cctcgagggg tggcccgtta
       61 cccaattcgc cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
      121 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
      181 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
      241 cgccctgtag cggcgcccgt ctccaggggg gctcctgtca gagcattcct ccaagcgtgc
      301 acagcttcca cagctgtcac tccgccgcgt ttagcgatct tcagcagctg tccagtgtcc
      361 agtccggact ggagtggtgg accccgcagt tccccggcga cggtgaggag tgcttcgagt
      421 gctctggcac cgctccactg ctttccgacg cccacaatgg cttcgtgggt tgcctcaggc
      481 agagcggcga tcatgtcctg atatttgacg gccacagtcc cgagtgcagc gggatgctga
      541 gacagagcca caatatgggc gtgtgtgaaa ccgtgcccca ccagtgcttc atggtgctga
      601 gcgacggtgg accgcacctt tggtttgatc ttctcctgct gctgctggct gtagccgagg
      661 gttctcagat ccacctgggc tgctggactg gcgggtaccc cacctccgcc actacctccg
      721 ccacctggtt tatcgtcgtc atccttgtag tcagcggccg ccaccttcct ctttttctta
      781 ggtcccatgg tggcggcccc tatagtgagt cgtattatta attaaggatc tgtaggaaaa
      841 agaagaaggc atgaacatgg ttagcagagg ctctagagcc gccggtcaca cgccagaagc
      901 cgaaccccgc cctgccccgt ccccc
//
