LOCUS       sequence-73134-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning E.coli expression vector with araBAD promoter, N-terminal
            10x His tag, 3C protease cleavage site and C-terminal Avi-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pBXNH3CA
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(89..95,96..113,114..119))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(144..268)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(287..307)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(311..331)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             358..402
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             412..441
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             457..474
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(530..547)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      700..786
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
       61 ctcgacggat cctaactcaa aatccacaca ttatacgagc cggaagcata aagtgtaaag
      121 cctggggtgc ctaatgcggc cgccatagtg actggatatg ttgtgtttta cagtattatg
      181 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
      241 ctcgttcagc ttttttgtac aaacttgtga tatcgaattc gctagctacc tttctcttct
      301 tttttggatc tacctttctc ttcttttttg gggatccact agttttgctc ttctgcaggt
      361 ctgaacgata tcttcgaagc tcagaaaatc gaatggcacg aataataact agaacaaaaa
      421 ctcatctcag aagaggatct gaatagcgcc gtcgaccatc atcatcatca tcattgagtt
      481 taaacggtct ccagcttggc tgttttggcg gatgagagaa gattttcagc ctgatacaga
      541 ttaaatcaga acgcagaagc ggtctgataa aacagaattt gcctggcggc agtagcgcgg
      601 tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg
      661 tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag
      721 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg
      781 acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac gc
//
