LOCUS       sequence-73143-e         225 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human codon optimized Cas9 nuclease from Streptococcus pyogenes 
            (Cbh-3X-FLAG-NLS-SpCas9-NLS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 225)
  AUTHORS   Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F
  TITLE     Genome engineering using the CRISPR-Cas9 system.
  JOURNAL   Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. 
            Epub 2013 Oct 24.
  PUBMED    24157548
REFERENCE   2  (bases 1 to 225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2013.143"; journalName: "Nat Protoc"; date: 
            "2013-11"; volume: "8"; issue: "11"; pages: "2281-308"
COMMENT     Sequence Label: pSpCas9 (PX165)
FEATURES             Location/Qualifiers
     source          1..225
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg
       61 gcgttactat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta
      121 ccgtaagtta tgtaacgggt acctctagag gccgcaggaa cccctagtga tggagttggc
      181 cactccctct ctgcgcgctc gctcgctcac agaggccggg cgacc
//
