LOCUS       sequence-73210-e        7717 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid with NR2E1 (Ple264) promoter driving expression of iCre.
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7717)
  AUTHORS   Korecki AJ, Hickmott JW, Lam SL, Dreolini L, Mathelier A, Baker O, 
            Kuehne C, Bonaguro RJ, Smith J, Tan CV, Zhou M, Goldowitz D, 
            Deussing JM, Stewart AF, Wasserman WW, Holt RA, Simpson EM
  TITLE     Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye 
            and Brain; Including Seventeen Carrying a New Inducible-First 
            Constitutive-Ready Allele.
  JOURNAL   Genetics. 2019 Feb 14. pii: genetics.119.301984. doi: 
            10.1534/genetics.119.301984.
  PUBMED    30765420
REFERENCE   2  (bases 1 to 7717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2019 Feb 14. pii: genetics.119.301984. doi: 
            10.1534/genetics.119.301984."
COMMENT     Sequence Label: pEMS1981
FEATURES             Location/Qualifiers
     source          1..7717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     intron          3274..3406
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3389..3408
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     polyA_signal    4487..4608
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4498..4517)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4552..4571
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   4778..4907
                     /label=AAV2 ITR
     primer_bind     complement(4926..4943)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4926..4942)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4935..4957)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5084..5539
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5171..5190)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5381..5402
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5565..5669
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5670..5738,5739..6530)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5888..5907)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6701..7289
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7190..7209
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    7577..7598
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7613..7618,7619..7636,7637..7643)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7651..7667
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7656..7678
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7675..7691
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7675..7691
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg gaattcccta ggccggccac gcgtgttgcc accggatgaa aggtggagga
      241 ggcagaaacc taaacaagtg aattcaatta catctgaaaa agattaggct aaattaggat
      301 tcattccttg ggccagttcc aggaaatatt tgtgaaaagt tggttgaaag aatactacca
      361 tctaaaccct aattttgcta ttacgtaaaa gccccgattc ctttgcagta aaacattaga
      421 aaatgttttc ctcattttgt ttttgctact ggtgtgtgac ctcttaattt aattttggtt
      481 tttgctcata taaatcaatg gccgtggaaa ctttttcctg tggtgcagca aagacagggg
      541 aaaagtagaa catcagtaat tatattttag gcacttaatt tatttaatga gcagttatca
      601 agcaccctta atggttccag ccataaacta taggctcctc ccgctccctc ggcctccagg
      661 ttcaataagg attacatcat ggagcaagta tgcggggcag cctgagaatc aaagccgtca
      721 gcaccggcgg ctggttaact gtactgttgc tgaaacacat ccttcccacc ccctccccgc
      781 gcccctctcc ctgccccggc cctctgcttt tcgtaccatt aaggatagca ttattgggga
      841 gaggaaaatc tgatcaaaac tgtcgaccgc caacgacaga ttttctatgg attctataaa
      901 ggccacctta acgatttccc taattaatgg atgacatctt tggctgaagg agaaacgatg
      961 gattcgactc tgtcttcgtc tttgttaacc ttctaattaa ctttcgtagg tcacccaggt
     1021 ctcccaaacc tgaaaagtga agaggcccat tgtgcgagga gcagtaaact tactttttct
     1081 gctggggtac ttaaaaggga gagcggctat aggccgagaa aaataactcc atttaaaagc
     1141 tttgtggctg cagagaatac ataggtccct cccttagaaa ataattgaaa caccttttat
     1201 accaatattt ctatgcataa taaaaacaaa ttagcaaaat gcatctaaat tcagcagcac
     1261 tttcaaatta ccttttttgg aatgtaagcc tctgcaagtc aacaaaagga gttgggttta
     1321 gagagaaaat tgcaactgca acagctatca gcctttatgc tgagagagca agttacacct
     1381 gaaattttag aggctaagga gaaatgtctg caacctacct cctcagttgt gaactcctgt
     1441 acctgaaagg ccaaatcctc ctcctcccag caggcaatca tcttatgccc tgaagcatga
     1501 gatctgatta cccctatcat catcttagct tgcatagata cagatgttat taacggctgt
     1561 agtttatcca accctttttt aaaacgcagc tgcagcattt gacttgggga cctcctgtga
     1621 atcccactgg ttgactccgt cttgcacaaa gcacgtgggg tttgggggag ggagaagaga
     1681 gagggaggtt tgttttgttt tggagggttt tttttttttt tttttgaatc agagttccct
     1741 aaatagccac tcggcatctt tgtaagtgat gtgaattctg aaattactta ctagaatagg
     1801 acaacagagt actgtgtgaa ctagatctgt tggaaacaaa gcatagtaga atgtatgatg
     1861 cttttcactt cattggcaga gatcaaatat gtggtcctga tatactcatc cacataataa
     1921 agaattgtag cttattctga aatatattgg ccacattcaa actggagtac ctggttttac
     1981 ataacaattg tttctacaga agaccccatc ctcagccctg actttccgta gatatgtgca
     2041 aaatgagtaa attactcacc tcgggccaga tccaagtttt acccaacaga aggggcaccg
     2101 gaccaagaat gaaccaactc acatggccat gtccggcgcg cacaatcaca cgccagcaca
     2161 cagccaccca atttcttccg cgaatctatc tggcactctg gagagagggg gaaaagcgtt
     2221 ttgagaaagc cccgtcaccc ctccccttcc ttcttgccgt gaaatatacg aattcatttt
     2281 tattacgagc cgcaccgtcc tcaccatcac gcacgcacag agccacactc ccatattcac
     2341 actttctaac tcgtaagctc cgacagcgcc tgcattttct ttgggagccg cttggaggtt
     2401 cattaatatc attagcattt aaccccctcc ctcttcccat cccctccccg cacatggctg
     2461 acgtcagacc ccgccaggag ttgggggaaa agctaagtgg gccagggacg ccctattccc
     2521 ctccccgcgg ctgcctgtca gagcgcttct ggagatatta caggggaccc agcccgcagc
     2581 gacaggcaca aagtcacggg gtaatgaact tcggggaccc ttcgccgctg cgtgcgcggc
     2641 tctccccgga aacccggacc tggccgcctc ttccctcgga agatttccca gcaatctagt
     2701 tttcccactc tgcgcttggg ttccggcagc gcggagcccg tctgcctctg agactgcggt
     2761 agtgttttcc ttctttcctt gggagaccag cggtcggcag agattgccca cactctgcat
     2821 gcctatgtag agggagagat cgaagactga gtgacaggaa tggggaaaaa gagggatttc
     2881 gctccgtagg aaggccattt tcgtgtctcc atctctgtct ttcaacatcc ctctcttgct
     2941 gttcttcctt cttcctcagt cttcctgtcc atctctccat ctgtctgtcc atgtgtgtgt
     3001 ccatatcaag cagcattccc agcagctgcg gttttgcaag agccgggaag aaacttaagg
     3061 atgcttaaat ttccactgtt ggacgaattc tgagcgccca gggagcagcg cagcgcgcga
     3121 ctgacaccca cctgtcccgc ccaggagcct tgcaggctgg agggcagctg gagagcggcg
     3181 gcgcccggcg gcgaggcggg cgctgccggc cgggactcgg gcagcgccca ccaaccgctg
     3241 gcgcgccgaa gttggtcgtg aggcactggg caggtaagta tcaaggttac aagacaggtt
     3301 taaggagacc aatagaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     3361 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgt ccactcccag
     3421 gcggccgcca ccatgtccaa cctgctgact gtgcaccaaa acctgcctgc cctccctgtg
     3481 gatgccacct ctgatgaagt caggaagaac ctgatggaca tgttcaggga caggcaggcc
     3541 ttctctgaac acacctggaa gatgctcctg tctgtgtgca gatcctgggc tgcctggtgc
     3601 aagctgaaca acaggaaatg gttccctgct gaacctgagg atgtgaggga ctacctcctg
     3661 tacctgcaag ccagaggcct ggctgtgaag accatccaac agcacctggg ccagctcaac
     3721 atgctgcaca ggagatctgg cctgcctcgc ccttctgact ccaatgctgt gtccctggtg
     3781 atgaggagaa tcagaaagga gaatgtggat gctggggaga gagccaagca ggccctggcc
     3841 tttgaacgca ctgactttga ccaagtcaga tccctgatgg agaactctga cagatgccag
     3901 gacatcagga acctggcctt cctgggcatt gcctacaaca ccctgctgcg cattgccgaa
     3961 attgccagaa tcagagtgaa ggacatctcc cgcaccgatg gtgggagaat gctgatccac
     4021 attggcagga ccaagaccct ggtgtccaca gctggtgtgg agaaggccct gtccctgggg
     4081 gttaccaagc tggtggagag atggatctct gtgtctggtg tggctgatga ccccaacaac
     4141 tacctgttct gccgggtcag aaagaatggt gtggctgccc cttctgccac ctcccaactg
     4201 tccacccggg ccctggaagg gatctttgag gccacccacc gcctgatcta tggtgccaag
     4261 gatgactctg ggcagagata cctggcctgg tctggccact ctgccagagt gggtgctgcc
     4321 agggacatgg ccagggctgg tgtgtccatc cctgaaatca tgcaggctgg tggctggacc
     4381 aatgtgaaca tagtgatgaa ctacatcaga aacctggact ctgagactgg ggccatggtg
     4441 aggctgctcg aggatgggga ctgagcggcc gcgatcgcag acatgataag atacattgat
     4501 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     4561 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     4621 tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta aagcaagtaa
     4681 aacctctaca aatgtggtag gtcgaaattc ccgataagga tcttcctaga gcatggctac
     4741 gtagataagt agcatggcgg gttaatcatt aactacaagg aacccctagt gatggagttg
     4801 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
     4861 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagcct taattaacct
     4921 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     4981 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     5041 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     5101 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     5161 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5221 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5281 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     5341 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     5401 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     5461 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     5521 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5581 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5641 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5701 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5761 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5821 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5881 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5941 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6001 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6061 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6121 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6181 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6241 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6301 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6361 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6421 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6481 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6541 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6601 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6661 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6721 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6781 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6841 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6901 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6961 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7021 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7081 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7141 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7201 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7261 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7321 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     7381 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     7441 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     7501 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     7561 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     7621 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     7681 acagctatga ccatgattac gccagattta attaagg
//
