LOCUS       sequence-73230-e        5672 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid with DBH promoter (PRS2/3:Con) driving expression of 
            iCre..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, 
            Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner 
            LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW
  TITLE     New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression
            in Retinal Ganglion Cells of Mouse and Primate Retina.
  JOURNAL   Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118.
  PUBMED    30062914
REFERENCE   2  (bases 1 to 5672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118."
COMMENT     Sequence Label: pEMS2022
FEATURES             Location/Qualifiers
     source          1..5672
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     CDS             388..399
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     intron          1230..1362
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1345..1364
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     polyA_signal    2443..2564
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2454..2473)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2508..2527
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   2733..2862
                     /label=AAV2 ITR
     primer_bind     complement(2881..2898)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2881..2897)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2890..2912)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3039..3494
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3126..3145)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3336..3357
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3520..3624
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3625..3693,3694..4485)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3843..3862)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4656..5244
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5145..5164
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5532..5553
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5568..5573,5574..5591,5592..5598)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5606..5622
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5611..5633
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5630..5646
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5630..5646
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg gaattcccta ggccggcctc tacttgtggg agaggacagg agggagaggt
      241 gccgtggtga gactgaccct cgggcccacg ggtggatgat ggcaaaggtc ctgtggcagg
      301 tgtgggagaa gcagggctgc ggccagctgc cctgagaggc ttcaaattca ggccagatct
      361 gctctgggca agaggaagct gtgggtttgg gagcttcaga gacaggggag caggcctgtc
      421 actcaccctc ttttccttaa aggctgtcac ccccagcagt gcaccctgca gcctgcctat
      481 cccctgtgca gctccagctc cgtctgtccc cagcaatgca ccccacgccc atgtccccca
      541 ctccctatga tgctctcgcg ccttctggag cagcagtggt accagctgga ggctgtggaa
      601 aaattgagag gagacaaaag tgggaagagc aggccgtgga gagagtgtct aaacacaggg
      661 actatttgga gattgtctta tttttggttt gaaatgggcc cacctgctag tattgtacaa
      721 acggctctgc tcgctaggcc aggcctgcag tggctacggg cctatccttc actggtcagc
      781 ctggagcagc tcctcggacc tcagtctgct catccgtggg tagaggcgac gcagacccca
      841 tcccactggg gactgggcca gacagcctgt gaggcagtca gctggtgcct ggccagaggg
      901 tgtctgaaag gcctcttggc tgcagggtgc atctgctttt gggacagctc ttcagagcca
      961 tctcagaagg gacagcatcc gcctgtctac ttcaactccc actgatgacg tccatgtgtc
     1021 attagtgcca attagaggag ggcagcaggc tgagtgcttg gcctggggcg caagcttgtg
     1081 ggagggataa ttggattacc cgctagacta atgtgattac ccgtgctgcc tggacccacc
     1141 ccattcagga ccagggcata aatggccagg tgggaccaga gagctcaccc cagccggcgc
     1201 gccgaagttg gtcgtgaggc actgggcagg taagtatcaa ggttacaaga caggtttaag
     1261 gagaccaata gaaactgggc ttgtcgagac agagaagact cttgcgtttc tgataggcac
     1321 ctattggtct tactgacatc cactttgcct ttctctccac aggtgtccac tcccaggcgg
     1381 ccgccaccat gtccaacctg ctgactgtgc accaaaacct gcctgccctc cctgtggatg
     1441 ccacctctga tgaagtcagg aagaacctga tggacatgtt cagggacagg caggccttct
     1501 ctgaacacac ctggaagatg ctcctgtctg tgtgcagatc ctgggctgcc tggtgcaagc
     1561 tgaacaacag gaaatggttc cctgctgaac ctgaggatgt gagggactac ctcctgtacc
     1621 tgcaagccag aggcctggct gtgaagacca tccaacagca cctgggccag ctcaacatgc
     1681 tgcacaggag atctggcctg cctcgccctt ctgactccaa tgctgtgtcc ctggtgatga
     1741 ggagaatcag aaaggagaat gtggatgctg gggagagagc caagcaggcc ctggcctttg
     1801 aacgcactga ctttgaccaa gtcagatccc tgatggagaa ctctgacaga tgccaggaca
     1861 tcaggaacct ggccttcctg ggcattgcct acaacaccct gctgcgcatt gccgaaattg
     1921 ccagaatcag agtgaaggac atctcccgca ccgatggtgg gagaatgctg atccacattg
     1981 gcaggaccaa gaccctggtg tccacagctg gtgtggagaa ggccctgtcc ctgggggtta
     2041 ccaagctggt ggagagatgg atctctgtgt ctggtgtggc tgatgacccc aacaactacc
     2101 tgttctgccg ggtcagaaag aatggtgtgg ctgccccttc tgccacctcc caactgtcca
     2161 cccgggccct ggaagggatc tttgaggcca cccaccgcct gatctatggt gccaaggatg
     2221 actctgggca gagatacctg gcctggtctg gccactctgc cagagtgggt gctgccaggg
     2281 acatggccag ggctggtgtg tccatccctg aaatcatgca ggctggtggc tggaccaatg
     2341 tgaacatagt gatgaactac atcagaaacc tggactctga gactggggcc atggtgaggc
     2401 tgctcgagga tggggactga gcggccgcga tcgcagacat gataagatac attgatgagt
     2461 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     2521 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     2581 ttcattttat gtttcaggtt cagggggaga tgtgggaggt tttttaaagc aagtaaaacc
     2641 tctacaaatg tggtagtcga aattcccgat aaggatcttc ctagagcatg gctacgtaga
     2701 taagtagcat ggcgggttaa tcattaacta caaggaaccc ctagtgatgg agttggccac
     2761 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
     2821 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agccttaatt aacctaattc
     2881 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     2941 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     3001 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     3061 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     3121 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     3181 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     3241 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     3301 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     3361 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     3421 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     3481 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     3541 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3601 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     3661 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     3721 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     3781 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     3841 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     3901 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     3961 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     4021 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     4081 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     4141 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     4201 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     4261 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     4321 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     4381 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     4441 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     4501 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     4561 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     4621 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     4681 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     4741 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     4801 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     4861 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     4921 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     4981 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     5041 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     5101 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     5161 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     5221 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     5281 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     5341 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     5401 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     5461 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     5521 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     5581 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     5641 tatgaccatg attacgccag atttaattaa gg
//
