LOCUS       sequence-73281-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning Xenopus oocyte expression vector with SP6 promoter and 
            N-terminal chicken alpha7 signal sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pTLNXA7
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(143..448)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(245..264)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 aacgctcaac tttggcagat cgcgagccac catgggactg agagctctga tgctgtggct
       61 gctggctgct gctggactgg tgagagaatc tctgagttga agagcgacct gcagactggc
      121 tgtgtataag ggagcctgac atttatattc cccagaacat caggttaatg gcgtttttga
      181 tgtcattttc gcggtggctg agatcagcca cttcttcccc gataacggag accggcacac
      241 tggccatatc ggtggtcatc atgcgccagc tttcatcccc gatatgcacc accgggtaaa
      301 gttcacggga gactttatct gacagcagac gtgcactggc cagggggatc accatccgtc
      361 gcccgggcgt gtcaataata tcactctgta catccacaaa cagacgataa cggctctctc
      421 ttttataggt gtaaacctta aactgcattt caccagcccc tgttctcgtc agcaaaagag
      481 ccgttcattt caataaaccg ggcgacctca gccatccctt cctgattttc cgctttccag
      541 cgttcggcac gcagacgacg ggcttcattc tgcatggttg tgcttaccag accggagata
      601 ttgacatcat atatgccttg agcaactgat agctgtcgct gtcaactgtc actgtaatac
      661 gctgcttcat agcatacctc tttttgacat acttcgggta tacatatcag tatatattct
      721 tataccgcaa aaatcagcgc gcaaatacgc atactgttat ctggctttta gtaagccgga
      781 tccacgcgtt tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
      841 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
      901 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga a
//
