LOCUS       sequence-73386-e         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning insect cell expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Weinert C, Morger D, Djekic A, Grutter MG, Mittl PR
  TITLE     Crystal structure of TRIM20 C-terminal coiled-coil/B30.2 fragment: 
            implications for the recognition of higher order oligomers.
  JOURNAL   Sci Rep. 2015 Jun 4;5:10819. doi: 10.1038/srep10819.
  PUBMED    26043233
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-06-4"; volume: "5:10819. doi"; pages: " 
            10.1038/srep10819"
COMMENT     Sequence Label: pFBXC3GH
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(159..464)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(261..280)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 aaaaacctat aaatattccg gattattcat accgtcccac catcgggcgc ggatcccggt
       61 ccgaagcgcg cggaattcaa aggcctacgt cgacgagctc atacaccatg agttgaagag
      121 cgacctgcag actggctgtg tataagggag cctgacattt atattcccca gaacatcagg
      181 ttaatggcgt ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata
      241 acggagaccg gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata
      301 tgcaccaccg ggtaaagttc acgggagact ttatctgaca gcagacgtgc actggccagg
      361 gggatcacca tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cacaaacaga
      421 cgataacggc tctctctttt ataggtgtaa accttaaact gcatttcacc agcccctgtt
      481 ctcgtcagca aaagagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg
      541 attttccgct ttccagcgtt cggcacgcag acgacgggct tcattctgca tggttgtgct
      601 taccagaccg gagatattga catcatatat gccttgagca actgatagct gtcgctgtca
      661 actgtcactg taatacgctg cttcatagca tacctctttt tgacatactt cgggtataca
      721 tatcagtata tattcttata ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg
      781 cttttagtaa gccggatcca cgcgtttacg ccccgccctg ccactcatcg cagtactgtt
      841 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
      901 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgccc
//
