LOCUS       sequence-7339-ex        9072 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9072)
  TITLE     Unpublished He lab plasmids
REFERENCE   2  (bases 1 to 9072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAd-RFP
FEATURES             Location/Qualifiers
     source          1..9072
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        416..719
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        720..923
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             954..1628
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             954..1628
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(1084..1102)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1352..1371
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1546..1569
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    1757..1878
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1794..1813)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1848..1867
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     enhancer        1948..2251
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2252..2455
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    2457..2475
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2478..2496
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2499..2517
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2520..2538
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(2693..2710)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2699..2923
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6292..6309)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6463..7051)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6543..6562)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             7864..8658
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7864..8658
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7918..7937)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8528..8547
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8768..8789)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8980..8999
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttaattaann ntcccttcca gctctctgcc ccttttggat tgaagccaat atgataatga
       61 gggggtggag tttgtgacgt ggcgcggggc gtgggaacgg ggcgggtgac gtagtagtgt
      121 ggcggaagtg tgatgttgca agtgtggcgg aacacatgta agcgacggat gtggcaaaag
      181 tgacgttttt ggtgtgcgcc ggtgtacaca ggaagtgaca attttcgcgc ggttttaggc
      241 ggatgttgta gtaaatttgg gcgtaaccga gtaagatttg gccattttcg cgggaaaact
      301 gaataagagg aagtgaaatc tgaataattt tgtgttactc atagcgcgta annngtagtt
      361 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
      421 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      481 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      541 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      601 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      661 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      721 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
      781 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      841 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      901 gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag accatggcct
      961 cctccgagga cgtcatcaag gagttcatgc gcttcaaggt gcgcatggag ggctccgtga
     1021 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     1081 ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc
     1141 ctcagttcca gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
     1201 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     1261 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
     1321 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
     1381 gggaggcctc caccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaaga
     1441 tgaggctgaa gctgaaggac ggcggccact acgacgccga ggtcaagacc acctacatgg
     1501 ccaagaagcc cgtgcagctg cccggcgcct acaagaccga catcaagctg gacatcacct
     1561 cccacaacga ggactacacc atcgtggaac agtacgagcg cgccgagggc cgccactcca
     1621 ccggcgccaa tccaccggat ctagataact gatcataatc agccatacca catttgtaga
     1681 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     1741 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     1801 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     1861 actcatcaat gtatcttaac gcgtctctag ttattaatag taatcaatta cggggtcatt
     1921 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     1981 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     2041 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     2101 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     2161 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     2221 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     2281 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     2341 gagtttgttt tggaaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     2401 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc
     2461 tatcagtgat agagatctcc ctatcagtga tagagatctc cctatcagtg atagagatct
     2521 ccctatcagt gatagagatc gagctgttta gtgaaccgtc agatcgcctg gagacgccat
     2581 ccacgctgtt ttgacctcca tagaagacac cgaattcagg ggatccggta ccgtcgacgc
     2641 ggccgcctcg agaagctttc tagagatatc ggccgctaaa cccgctgatc agcctcgact
     2701 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     2761 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     2821 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     2881 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     2941 accaggatct gctaggatct nnntaagggt gggaaagaat atataaggtg ggggtcttat
     3001 gtagttttgt atctgttttg cagcagccgc cgccgccatg agcaccaact cgtttgatgg
     3061 aagcattgtg agctcatatt tgacaacgcg catgccccca tgggccgggg tgcgtcagaa
     3121 tgtgatgggc tccagcattg atggtcgccc cgtcctgccc gcaaactcta ctaccttgac
     3181 ctacgagacc gtgtctggaa cgccgttgga gactgcagcc tccgccgccg cttcagccgc
     3241 tgcagccacc gcccgcggga ttgtgactga ctttgctttc ctgagcccgc ttgcaagcag
     3301 tgcagcttcc cgttcatccg cccgcgatga caagttgacg gctcttttgg cacaattgga
     3361 ttctttgacc cgggaactta atgtcgtttc tcagcagctg ttggatctgc gccagcaggt
     3421 ttctgccctg aaggcttcct cccctcccaa tgcggtttaa aacataaata aaaaaccaga
     3481 ctctgtttgg atttggatca agcaagtgtc ttgctgtctt tatttagggg ttttgcgcgc
     3541 gcggtaggcc cgggaccagc ggtctcggtc gttgagggtc ctgtgtattt tttccaggac
     3601 gtggtaaagg tgactctgga tgttcagata catgggcata agcccgtctc tggggtggag
     3661 gtagcaccac tgcagagctt catgctgcgg ggtggtgttg tagatgatcc agtcgtagca
     3721 ggagcgctgg gcgtggtgcc taaaaatgtc tttcagtagc aagctgattg ccaggggcag
     3781 gcccttggtg taagtgttta caaagcggtt aagctgggat gggtgcatac gtggggatat
     3841 gagatgcatc ttggactgta tttttaggtt ggctatgttc ccagccatat ccctccgggg
     3901 attcatgttg tgcagaacca ccagcacagt gtatccggtg cacttgggaa atttgtcatg
     3961 tagcttagaa ggaaatgcgt ggaagaactt ggagacgccc ttgtgacctc caagattttc
     4021 catgcattcg tccataatga tggcaatggg cccacgggcg gcggcctggg cgaagatatt
     4081 tctgggatca ctaacgtcat agttgtgttc caggatgaga tcgtcatagg ccatttttac
     4141 aaagcgcggg cggagggtgc cagactgcgg tataatggtt ccatccggcc caggggcgta
     4201 gttaccctca cagatttgca tttcccacgc tttgagttca gatgggggga tcatgtctac
     4261 ctgcggggcg atgaagaaaa cggtttccgg ggtaggggag atcagctggg aagaaagcag
     4321 gttcctgagc agctgcgact taccgcagcc ggtgggcccg taaatcacac ctattaccgg
     4381 gtgcaactgg tagttaagag agctgcagct gccgtcatcc ctgagcaggg gggccacttc
     4441 gttaagcatg tccctgactc gcatgttttc cctgaccaaa tccgccagaa ggcgctcgcc
     4501 gcccagcgat agcagttctt gcaaggaagc aaagtttttc aacggtttga gaccgtccgc
     4561 cgtaggcatg cttttgagcg tttgaccaag cagttccagg cggtcccaca gctcggtcac
     4621 ctgctctacg gcatctcgat ccagcatatc tcctcgtttc gcgggttggg gcggctttcg
     4681 ctgtacggca gtagtcggtg ctcgtccaga cgggccaggg tcatgtcttt ccacgggcgc
     4741 agggtcctcg tcagcgtagt ctgggtcacg gtgaaggggt gcgctccggg ctgcgcgctg
     4801 gccagggtgc gcttgaggct ggtcctgctg gtgctgaagc gctgccggtc ttcgccctgc
     4861 gcgtcggcca ggtagcattt gaccatggtg tcatagtcca gcccctccgc ggcgtggccc
     4921 ttggcgcgca gcttgccctt ggaggaggcg ccgcacgagg ggcagtgcag acttttgagg
     4981 gcgtagagct tgggcgcgag aaataccgat tccggggagt aggcatccgc gccgcaggcc
     5041 ccgcagacgg tctcgcattc cacgagccag gtgagctctg gccgttcggg gtcaaaaacc
     5101 aggtttcccc catgcttttt gatgcgtttc ttacctctgg tttccatgag ccggtgtcca
     5161 cgctcggtga cgaaaaggct gtccgtgtcc ccgtatacag actnnngttt aaacgaattc
     5221 nnntataaaa tgcaaggtgc tgctcaaaaa atcaggcaaa gcctcgcgca aaaaagaaag
     5281 cacatcgtag tcatgctcat gcagataaag gcaggtaagc tccggaacca ccacagaaaa
     5341 agacaccatt tttctctcaa acatgtctgc gggtttctgc ataaacacaa aataaaataa
     5401 caaaaaaaca tttaaacatt agaagcctgt cttacaacag gaaaaacaac ccttataagc
     5461 ataagacgga ctacggccat gccggcgtga ccgtaaaaaa actggtcacc gtgattaaaa
     5521 agcaccaccg acagctcctc ggtcatgtcc ggagtcataa tgtaagactc ggtaaacaca
     5581 tcaggttgat tcatcggtca gtgctaaaaa gcgaccgaaa tagcccgggg gaatacatac
     5641 ccgcaggcgt agagacaaca ttacagcccc cataggaggt ataacaaaat taataggaga
     5701 gaaaaacaca taaacacctg aaaaaccctc ctgcctaggc aaaatagcac cctcccgctc
     5761 cagaacaaca tacagcgctt cacagcggca gcctaacagt cagccttacc agtaaaaaag
     5821 aaaacctatt aaaaaaacac cactcgacac ggcaccagct caatcagtca cagtgtaaaa
     5881 aagggccaag tgcagagcga gtatatatag gactaaaaaa tgacgtaacg gttaaagtcc
     5941 acaaaaaaca cccagaaaac cgcacgcgaa cctacgccca gaaacgaaag ccaaaaaacc
     6001 cacaacttcc tcaaatcgtc acttccgttt tcccacgtta cgtaacttcc cattttaaga
     6061 aaactacaat tcccaacaca tacaagttac tccgccctaa aacctacgtc acccgccccg
     6121 ttcccacgcc ccgcgccacg tcacaaactc caccccctca ttatcatatt ggcttcaatc
     6181 caaaataagg tatattattg atgatnnntt aattaaggat cgatccnnnc ggtgtgaaat
     6241 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
     6301 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     6361 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     6421 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6481 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6541 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     6601 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6661 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6721 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6781 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6841 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6901 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     6961 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7021 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7081 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7141 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     7201 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     7261 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     7321 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     7381 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     7441 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     7501 gccagttaat agtttgcgca acgttgttgn nnnnnaaaaa ggatcttcac ctagatcctt
     7561 ttcacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctactgg
     7621 gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg cagtgggctt
     7681 acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga attgccagct
     7741 ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttctcgccg
     7801 ccaaggatct gatggcgcag gggatcaagc tctgatcaag agacaggatg aggatcgttt
     7861 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     7921 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     7981 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     8041 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     8101 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     8161 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     8221 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     8281 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     8341 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc
     8401 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     8461 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     8521 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     8581 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     8641 cttgacgagt tcttctgaat tttgttaaaa tttttgttaa atcagctcat tttttaacca
     8701 ataggccgaa atcggcaaca tcccttataa atcaaaagaa tagaccgcga tagggttgag
     8761 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     8821 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccca aatcaagttt
     8881 tttgcggtcg aggtgccgta aagctctaaa tcggaaccct aaagggagcc cccgatttag
     8941 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     9001 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgcgcg
     9061 cttaatgcgc cg
//
