LOCUS       sequence-73443-e        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   de Felippes FF, Wang JW, Weigel D
  TITLE     MIGS: MiRNA Induced Gene Silencing.
  JOURNAL   Plant J. 2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x.
  PUBMED    22211571
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x."
COMMENT     Sequence Label: MIGS3.2 (FF574)
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(106..112,113..130,131..136))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(161..285)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(310..655)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(347..367)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
       61 ccttagctcc tgaaaatctc gacggctcct aactcaaaat ccacacatta tacgagccgg
      121 aagcataaag tgtaaagcct ggggtgccta atggggccgc caatatgact ggatatgttg
      181 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
      241 tatatcattt tacgtttctc gttcagcttt tttgtacaaa cttgtgatcc tctagaagct
      301 cgtcccccgt gttctctcca aatgaaatga acttccttat atagaggaag ggtcttgcga
      361 aggatagtgg gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg
      421 ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc
      481 atctttggga ccactgtcgg cagaggcatc ttcaacgatg gcctttcctt tatcgcaatg
      541 atggcatttg taggagccac cttccttttc cactatcttc acaataaagt gacagatagc
      601 tgggcaatgg aatccgagga ggtttccgga tattaccctt tgttgaaaag tctcaattgc
      661 cctttggtct tctgagactg tatctttgat atttttggag tagacaagtg tgtcgtgctc
      721 caccatgttg acgaagattt tcttcttgtc attgagtcgt aagagactct gtatgaactg
      781 ttcgccagtc tttacggcga gttctgttag gtcctctatt tgaatctttg actccatggc
      841 ctttgattca gtgggaacta cctttttaga gactccaatc tctattactt gccttggttt
      901 gtgaagcaag ccttgaatcg tccatactgg aatagtactt ctgatcttga gaaatatatc
      961 tttctctgtg ttcttgatgc agttagtcct gaatcttttg actgcatctt taaccttctt
     1021 gggaaggtat ttgat
//
