LOCUS       sequence-73470-e         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Deuschle K, Okumoto S, Fehr M, Looger LL, Kozhukh L, Frommer WB
  TITLE     Construction and optimization of a family of genetically encoded 
            metabolite sensors by semirational protein engineering.
  JOURNAL   Protein Sci. 2005 Sep . 14(9):2304-14.
  PUBMED    16131659
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2005 Sep . 14(9):2304-14."
COMMENT     Sequence Label: pRSET FLII12Pglu-600u
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(377..398)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(638..657)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agtggttatc gccaacaacg atgcgatggc aatgggcgcg gttgaagcgc tgaaagcaca
       61 caacaagtcc agcattccgg tgtttggcgt cgatgcgctg ccagaagcgc tggcgctggt
      121 gaaatccggt gcactggcgg gcaccgtact gaacgatgct aacaaccagg cgaaagcgac
      181 ctttgatctg gcgaaaaacc tggccgatgg taaaggtgcg gctgatggca ccaactggaa
      241 aatcgacaac aaagtggtcc gcgtacctta tgttggcgta gataaagaca acctggctga
      301 attcagcaag aaaggcgccg gtaccggtgg aatggtgagc aagggcgagg agctgttcac
      361 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      421 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      481 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgat
      541 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      601 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      661 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      721 cttcaaggag gacggcaaca tcctggggca caagctggag tacaact
//
