LOCUS       sequence-73583-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway adapted transfer of Hygromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Xu D, Brandan CP, Basombrio MA, Tarleton RL
  TITLE     Evaluation of high efficiency gene knockout strategies for 
            Trypanosoma cruzi.
  JOURNAL   BMC Microbiol. 2009 May 11;9:90. doi: 10.1186/1471-2180-9-90.
  PUBMED    19432966
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Microbiol."; date: "2009-05-11"; volume: "9:90. doi"; pages: " 
            10.1186/1471-2180-9-90"
COMMENT     Sequence Label: pDONR221-Hyg
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
       61 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
      121 ctcgtccgag ggcaaaggaa tagagtagat gccgaccggg atccactagt cgacctcgag
      181 ccatttacga ctccaaggca acgctgcaga acaacctgcc gaaggagcgc cgcttcttca
      241 agattgtgtc gtggtacgac aacgagtggg gatactccca ccgcgtggtg gaccttgtac
      301 gccacatggc ctcgaaggat cgttcggcaa ggttgtaggc gtggcgatga cttcaggtct
      361 ttcttttgcg aatagggatc ttataataca cgatgcgtgt cccgtgatga tcgttaccgg
      421 tgctgccacg atccaattga cacagcgtca agagcaaaac aattttactt ttccctttaa
      481 ggacaacaac aaaaaaatat ataacttttt tttctttttt tttttttttg aaattatatt
      541 tatggtcatc tttgggaaac aaaaagcagc aatttaatga tgcggaagga tgagtgaaat
      601 aatgtttaat caatgtacga ggatttgggg tattgcaagg aaaatgtaga tgatttaatt
      661 gggtgtgtga tgcagcttgt ggtaattttt gctcacttcc ctttttgcca catcttttta
      721 gtttttctgc ttttctttcc ccattattcc acttgtctct cttttcccac gtttcctgca
      781 cgaatgcaga aagtgatatt tttactttga aagccatcta ccaacaacaa ttacattgaa
      841 tagaatttga cccagctttc t
//
