LOCUS       sequence-73589-e         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial nuclease-null SP Cas9 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: DS-SPcasN-
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gattatcgaa cttccgttgt gtgatcagtt tggcgttcag cagctgccgc caataatttt
       61 tcattttctt gacaacttct tctgagggga cgttatcact cttccctcta gctttatcgg
      121 atcttgtcaa cactttatta tcaatagaat catctttgag aaaagactgg ggcacgatag
      181 cagccacgtc gtagtcggag agccgattga tgtccagttc ctgatccacg tacatgtccc
      241 tgccgttctg caggtagtac aggtagagct tctcattctg aagctgggtg ttttcaactg
      301 ggtgttcctt aaggatttgg gaccccagtt cttttatacc ctcttcaatc ctcttcatcc
      361 tttccctact gttcttctgt cccttctggg tagtttggtt ctctcgggcc atctcgataa
      421 cgatattctc gggcttatgc cttcccatta ctttgacgag ttcatccacg accttaacgg
      481 tctgcagtat tccctttttg atagctgggc tacctgcaag attagcgatg tgctcgtgaa
      541 gactgtcccc ctggccagaa acttgtgctt tctggatgtc ctccttaaag gtgagagagt
      601 catcatggat caactgcatg aagttccggt tggcaaatcc atcggactta agaaaatcca
      661 ggattgtctt tccactctgc ttgtctcgga tcccattgat cagttttctt gacagccgcc
      721 cccatcctgt atatcggcgc ctcttgagct gtttcatgac tttgtcgtcg aagagatgag
      781 cgtaagtttt caagcgttct tcaatcatct ccctatcttc aaacaacgta agggtgagga
      841 caatgtcctc aagaatgtcc tcgttctcct cattgtccag gaagtccttg tctttaatga
      901 ttttcaggag atcgtgatac gttcccaggg atgcgttgaa gcgatcctcc actccgctga
      961 tttcaacaga gtcgaaacat
//
