LOCUS       sequence-73623-e        5469 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid expresses full-length human TET3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Ko M, An J, Bandukwala HS, Chavez L, Aijo T, Pastor WA, Segal MF, Li
            H, Koh KP, Lahdesmaki H, Hogan PG, Aravind L, Rao A
  TITLE     Modulation of TET2 expression and 5-methylcytosine oxidation by the 
            CXXC domain protein IDAX.
  JOURNAL   Nature. 2013 May 2;497(7447):122-6. doi: 10.1038/nature12052. Epub 
            2013 Apr 7.
  PUBMED    23563267
REFERENCE   2  (bases 1 to 5469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12052"; journalName: "Nature"; date: "2013-05-2- 2"; 
            volume: "497"; issue: "7447"; pages: "122-6"
COMMENT     Sequence Label: FH-TET3-pEF
FEATURES             Location/Qualifiers
     source          1..5469
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             40..66
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             40..66
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    2839..2850
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 atggactaca aggacgacga tgacaagctc gatggaggat acccctacga cgtgcccgac
       61 tacgccggag gactcgacag ccagtttcag gtgcccctgg ccgtccagcc ggacctgcca
      121 ggcctttatg acttccctca gcgccaggtg atggtaggga gcttcccggg gtctgggctc
      181 tccatggctg ggagtgagtc ccaactccga gggggtggag atggtcgaaa gaaacggaaa
      241 cggtgtggta cttgtgagcc ctgccggcgg ctggaaaact gtggcgcttg cactagctgt
      301 accaaccgcc gcacgcacca gatctgcaaa ctgcgaaaat gtgaggtgct gaagaaaaaa
      361 gtagggcttc tcaaggaggt ggaaataaag gctggtgaag gagccgggcc gtggggacaa
      421 ggagcggctg tcaagacagg ctcagagctc agcccagttg atggacctgt tccaggtcag
      481 atggactcag ggccagtgta ccatggggac tcacggcagc taagcgcctc aggggtgccg
      541 gtcaatggtg ctagagagcc cgctggaccc agtctgctgg ggactggggg tccttggcgg
      601 gtagaccaaa agcccgactg ggaggctgcc ccaggcccag ctcatactgc tcgcctggaa
      661 gatgcccacg atctggtggc cttttcggct gtggccgaag ctgtgtcctc ttatggggcc
      721 cttagcaccc ggctctatga aaccttcaac cgtgagatga gtcgtgaggc tgggaacaac
      781 agcaggggac cccggccagg gcctgagggc tgctctgctg gcagcgaaga ccttgacaca
      841 ctgcagacgg ccctggccct cgcgcggcat ggtatgaaac cacccaactg caactgcgat
      901 ggcccagaat gccctgacta cctcgagtgg ctggagggga agatcaagtc tgtggtcatg
      961 gaaggagggg aggagcggcc caggctccca gggcctctgc ctcctggtga ggccggcctc
     1021 ccagcaccaa gcaccaggcc actcctcagc tcagaggtgc cccagatctc tccccaagag
     1081 ggcctgcccc tgtcccagag tgccctgagc attgccaagg aaaaaaacat cagcttgcag
     1141 accgccattg ccattgaggc cctcacacag ctctcctctg ccctcccgca gccttctcat
     1201 tccacccccc aggcttcttg cccccttcct gaggccttgt cacctcctgc ccctttcaga
     1261 tctccccagt cttacctccg ggctccctca tggcctgtgg ttcctcctga agagcactca
     1321 tcttttgctc ctgatagctc tgccttccct ccagcaactc ctagaactga gttccctgaa
     1381 gcctggggca ctgacacccc tccagcaacg ccccggagct cctggcccat gcctcgccca
     1441 agccccgatc ccatggctga actggagcag ttgttgggca gcgccagtga ttacatccag
     1501 tcagtattca agcggcctga ggccctgcct accaagccca aggtcaaggt ggaggcaccc
     1561 tcttcctccc cggccccggc cccatcccct gtacttcaga gggaggctcc cacgccatcc
     1621 tcggagcccg acacccacca gaaggcccag accgccctgc agcagcacct ccaccacaag
     1681 cgcagcctct tcctagaaca ggtgcacgac acctccttcc ctgctccttc agagccttct
     1741 gctcctggct ggtggccccc accaagttca cctgtcccac ggcttccaga cagaccaccc
     1801 aaggagaaga agaagaagct cccaacacca gctggaggtc ccgtgggaac ggagaaagct
     1861 gcccctggga tcaagcccag tgtccgaaag cccattcaga tcaagaagtc caggccccgg
     1921 gaagcacagc ccctcttccc acctgtccga cagattgtcc tggaagggct taggtcccca
     1981 gcctcccagg aagtgcaggc tcatccaccg gcccctctgc ctgcctcaca gggctctgct
     2041 gtgcccctgc ccccagaacc ttctcttgcg ctatttgcac ctagtccctc cagggacagc
     2101 ctgctgcccc ctactcagga aatgaggtcc cccagcccca tgacagcctt gcagccaggc
     2161 tccactggcc ctcttccccc tgccgatgac aagctggaag agctcatccg gcagtttgag
     2221 gctgaatttg gagatagctt tgggcttccc ggcccccctt ctgtgcccat tcaggacccc
     2281 gagaaccagc aaacatgtct cccagcccct gagagcccct ttgctacccg ttcccccaag
     2341 caaatcaaga ttgagtcttc gggggctgtg actgtgctct caaccacctg cttccattca
     2401 gaggagggag gacaggaggc cacacccacc aaggctgaga acccactcac acccaccctc
     2461 agtggcttct tggagtcacc tcttaagtac ctggacacac ccaccaagag tctgctggac
     2521 acacctgcca agagagccca ggccgagttc cccacctgcg attgcgtcga acaaatagtg
     2581 gagaaagatg aaggtccata ttatactcac ttgggatctg gccccacggt cgcctctatc
     2641 cgggaactca tggaggagcg gtatggagag aaggggaaag ccatccggat cgagaaggtc
     2701 atctacacgg ggaaggaggg aaagagctcc cgcggttgcc ccattgcaaa gtgggtgatc
     2761 cgcaggcaca cgctggagga gaagctactc tgcctggtgc ggcaccgggc aggccaccac
     2821 tgccagaacg ctgtgatcgt catcctcatc ctggcctggg agggcattcc ccgtagcctc
     2881 ggagacaccc tctaccagga gctcaccgac accctccgga agtatgggaa ccccaccagc
     2941 cggagatgcg gcctcaacga tgaccggacc tgcgcttgcc aaggcaaaga ccccaacacc
     3001 tgtggtgcct ccttctcctt tggttgttcc tggagcatgt acttcaacgg ctgcaagtat
     3061 gctcggagca agacacctcg caagttccgc ctcgcagggg acaatcccaa agaggaagaa
     3121 gtgctccgga agagtttcca ggacctggcc accgaagtcg ctcccctgta caagcgactg
     3181 gcccctcagg cctatcagaa ccaggtgacc aacgaggaaa tagcgattga ctgccgtctg
     3241 gggctgaagg aaggacggcc cttcgcgggg gtcacggcct gcatggactt ctgtgcccac
     3301 gcccacaagg accagcataa cctctacaat gggtgcaccg tggtctgcac cctgaccaag
     3361 gaagacaatc gctgcgtggg caagattccc gaggatgagc agctgcatgt tctccccctg
     3421 tacaagatgg ccaacacgga tgagtttggt agcgaggaga accagaatgc aaaggtgggc
     3481 agcggagcca tccaggtgct caccgccttc ccccgcgagg tccgacgcct gcccgagcct
     3541 gccaagtcct gccgccagcg gcagctggaa gccagaaagg cagcagccga gaagaagaag
     3601 attcagaagg agaagctgag cactccggag aagatcaagc aggaggccct ggagctggcg
     3661 ggcattacgt cggacccagg cctgtctctg aagggtggat tgtcccagca aggcctgaag
     3721 ccctccctca aggtggagcc gcagaaccac ttcagctcct tcaagtacag cggcaacgcg
     3781 gtggtggaga gctactcggt gctgggcaac tgccggccct ccgaccctta cagcatgaac
     3841 agcgtgtact cctaccactc ctactatgca cagcccagcc tgacctccgt caatggcttc
     3901 cactccaagt acgctctccc gtcttttagc tactatggct ttccatccag caaccccgtc
     3961 ttcccctctc agttcctggg tcctggtgcc tgggggcata gtggcagcag tggcagtttt
     4021 gagaagaagc cagacctcca cgctctgcac aacagcctga gcccggccta cggtggtgct
     4081 gagtttgccg agctgcccag ccaggctgtt cccacagacg cccaccaccc cactcctcac
     4141 caccagcagc ctgcgtaccc aggccccaag gagtatctgc ttcccaaggc ccccctactc
     4201 cactcagtgt ccagggaccc ctcccccttt gcccagagct ccaactgcta caacagatcc
     4261 atcaagcaag agccagtaga cccgctgacc caggctgagc ctgtgcccag agacgctggc
     4321 aagatgggca agacacctct gtccgaggtg tctcagaatg gaggacccag tcacctttgg
     4381 ggacagtact caggaggccc aagcatgtcc cccaagagga ctaacggtgt gggtggcagc
     4441 tggggtgtgt tctcgtctgg ggagagtcct gccatcgtcc ctgacaagct cagttccttt
     4501 ggggccagct gcctggcccc ttcccacttc acagatggcc agtgggggct gttccccggt
     4561 gaggggcagc aggcagcttc ccactctgga ggacggctgc gaggcaaacc gtggagcccc
     4621 tgcaagtttg ggaacagcac ctcggccttg gctgggccca gcctgactga gaagccgtgg
     4681 gcgctggggg caggggattt caactcggcc ctgaaaggta gtcctgggtt ccaagacaag
     4741 ctgtggaacc ccatgaaagg agaggagggc aggattccag ccgcaggggc cagccagctg
     4801 gacagggcct ggcagtcctt tggtctgccc ctgggatcca gcgagaagct gtttggggct
     4861 ctgaagtcag aggagaagct gtgggacccc ttcagcctgg aggaggggcc ggctgaggag
     4921 ccccccagca agggagcggt gaaggaggag aagggcggtg gtggtgcgga ggaggaagag
     4981 gaggagctgt ggtcggacag tgaacacaac ttcctggacg agaacatcgg cggcgtggcc
     5041 gtggccccag cccacggctc catcctcatc gagtgtgccc ggcgggagct gcacgccacc
     5101 acgccgctta agaagcccaa ccgctgccac cccacccgca tctcgctggt cttctaccag
     5161 cacaagaacc tcaaccagcc caaccacggg ctggccctct gggaagccaa gatgaagcag
     5221 ctggcggaga gggcacgggc acggcaggag gaggctgccc ggctgggcct gggccagcag
     5281 gaggccaagc tctacgggaa gaagcgcaag tgggggggca ctgtggttgc tgagccccag
     5341 cagaaagaga agaagggggt cgtccccacc cggcaggcac tggctgtgcc cacagactcg
     5401 gcggtcaccg tgtcctccta tgcctacacg aaggtcactg gcccctacag ccgctggatc
     5461 tagtctaga
//
