LOCUS       sequence-73670-e       10432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overespression of Flp step-arrest mutant in yeast, LYS2 selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10432)
  TITLE     Gartenberg plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMC6-TEFkFLP(LYS2)
FEATURES             Location/Qualifiers
     source          1..10432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    40..543
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     599..617
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(655..677)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     777..796
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(1194..5372)
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     promoter        complement(5373..5612)
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     rep_origin      complement(5666..6121)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5803..5824)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6015..6034
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6251..6273
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6265..6282
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6266..6282
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6293..6311
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     6293..6310
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        6424..6767
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             6768..8039
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MPQFGILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSLIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     primer_bind     complement(8267..8287)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8267..8285)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8306..8322)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8306..8322)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8319..8341)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8330..8346
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8354..8360,8361..8378,8379..8384))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(8537..8554)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8708..9296)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(9467..10258,10259..10327))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10090..10109
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10328..10432)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctggtcg gtccttttca tcacgtgcta taaaaataat
       61 tataatttaa attttttaat ataaatatat aaattaaaaa tagaaagtaa aaaaagaaat
      121 taaagaaaaa atagtttttg ttttccgaag atgtaaaaga ctctaggggg atcgccaaca
      181 aatactacct tttatcttgc tcttcctgct ctcaggtatt aatgccgaat tgtttcatct
      241 tgtctgtgta gaagaccaca cacgaaaatc ctgtgatttt acattttact tatcgttaat
      301 cgaatgtata tctatttaat ctgcttttct tgtctaataa atatatatgt aaagtacgct
      361 ttttgttgaa attttttaaa cctttgttta tttttttttc ttcattccgt aactcttcta
      421 ccttctttat ttactttcta aaatccaaat acaaaacata aaaataaata aacacagagt
      481 aaattcccaa attattccat cattaaaaga tacgaggcgc gtgtaagtta caggcaagcg
      541 atccgtcgat ccgtcgacct gctaagaaac cattattatc atgacattaa cctataaaaa
      601 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
      661 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
      721 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
      781 atcagagcag attgtactga gagtgcacca tatagcttcg caagtattca ttttagaccc
      841 atggtggaac cctagtgttg aatggcaaag tggtgataga gttcatagaa ttggtcagta
      901 tcgacctgtg aagatcacaa ggttttgcat tgaggatagt atagaagcaa gaatcattga
      961 attacaggaa aaaaaggcaa atatgattca tgctacaata aaccaagatg aagctgccat
     1021 tagcagacta acgccagctg atttacagtt cttattcaat aactaatatt ttattctctt
     1081 attatatatt attctcggag tttttaagtg acatcacccg aaaagaagct aagtctttct
     1141 cctaattcat atttaattat tgtacatgga catatcatac gtaatgctca accttaagct
     1201 gctgcggagc ttccacgagc accagcacct gaagcaacta gacttatttg cgcttgagtt
     1261 agttctatac ttggcagtgg aagtttgtca ttatgagttg gtggaggtaa aaatccaacc
     1321 ttgtttaaaa atgcaatata tataccaacc tcttctggag taacacctat tccattagac
     1381 caatcaacac ctgtccatgc ggtgtctttc tttaaagatg ccacggcgtt cctatcgtct
     1441 agttccggag ctttggtact ttcaggtaag ttgtctaaga ccatgtgtag caaaggatac
     1501 aacgcatttt cttcattcct gtcaataaca gacgcctcca atgatttctt ccatttagaa
     1561 tagctttcga tttcgacatc gtaaccataa tcgtgtaaag tatacaagta gtctttgaat
     1621 aatattcttg ggtgacccgt tacttgagca acggccaatt cattttcttt gggaggattc
     1681 aaagacgtag caacaacaac acgagcaaca tgatctactg gaaccatatt cacggaattt
     1741 tcgatatctg gaatcttacc taattggact gaacctttca aaaatctcaa taagaaatca
     1801 tctgtgtttg aagaaccatt ggcagaggca cctgttacgt aacctggtct gacaatacac
     1861 ccacgtaggc ccctttcacc tgcacgtcta atgatgtact cagcagccca tttggactga
     1921 ccatatccac cagtgagccc gcttgcagag ttcattaaat cgtctgattc taaaatgcct
     1981 ggcttccctt cgctaacaag tttatctgac aaattaaagt agtattcagt gtcaagagta
     2041 gaagtggagg aaacaaagtc aaagaacttt ggcttgccga cggcggctaa gctcataaca
     2101 ttgatagttg aaataacatt tggatccctc aatttggcat atggataaac ccagtgaact
     2161 aacgcaccat tatggataat tatatcaact gtgtttgcca aatccatcca cttctcatct
     2221 gaaagaccaa attggctttt agataaatcg cctaatacaa ctttaatatt tgaggcaaat
     2281 ttttcgttcc aagtaccata ggtgatacct gccttttgta atcttgcaaa tgcagcttct
     2341 tcatccttgg ccctgacgtg ggcaaacact ttgaaactgt agttctttgg agaacgtcct
     2401 aacaaatctg caaggatgta ggagcccaga aatcctgtga caccggtaac aaacacatta
     2461 attgttgttt ttccttcggc actattaggt tcaacaaaat attctcgaga ggggtacgaa
     2521 cttggtagcg tctcaaccaa tttcttggcg tcttccgcat aatttgcagt gacattttcg
     2581 acgacctcac cttgagatga tccacccgat gatttaattc tgtcaatttc cgcggcaaag
     2641 gcctttatcg ttggatactt gaaaattgtg cccaatggta aatcaacttg cagctttttc
     2701 tttaaggtaa aaatcatttt ggtagccaag atagaatgac cacctaaatc gaaaaacgaa
     2761 tcatctggtg atacagatgc tggcttggta ggtaatatac ttaaccataa gtctctaacc
     2821 tcgcgctcaa cattggtaaa ctgagagtcg tcagtttcag aaactgtatt ttcagctacc
     2881 aaatttaatt gcttgggagt tgggaattga agtttaggct tatcaacttt accatttgga
     2941 ttcaatggta gtttatccat aaccacaatc aaggaaggca tagcatagct agccaatctt
     3001 ttctttaaga aagtcctgat gtccttggat aaaagatggt aaccgattaa gcccttaact
     3061 atagggtcag tttcaacctc ctttggaaca tcactttgga acttagacaa gtcatctggc
     3121 ttgtcaaatc ttgggaccat aaatgtgatc aatgttggct cattgtcggc atttttgcga
     3181 actaaagtaa tgttttctct taccaatgga tgttgggaaa tgtgcgtatc tatttctcct
     3241 aattcgattc tgaacccacg aattttaact tgatcatcag ccctaccgca acattcacag
     3301 tcaccgtttg gtagataacg acctaaatca cccgttctgt acaatctatc tcttggacct
     3361 aaccagaatt gtctccaagg ttcaccatta tccttatcca aataattcca gtgatctttt
     3421 tcaacaaacc agttgttcac aaatttttct ttattcaatt ctggtaatcc tctataacct
     3481 tcggccaaac cacctgcacg aacataaatc tcacctattt cgccaatacc acatatttga
     3541 gtacgatcgt tcctgttaac aactagtagc tgaacgttca acataccttt accagcaggc
     3601 atgacatctt tcaatttttt caaaaagttt ggatcgtcat tttttgattt aacttcgaaa
     3661 taagaaactg cacgctgtgt ttcagtggta ccgtacatat taacaatacg gcaattttct
     3721 gccaaggttt gtaacctcag acaatcacgt tttgttaaaa tgtcacccac aaagaacgca
     3781 tgatgtaact tagggaatgg tgtagtagct tgggcagtaa gtaattgacc catggcaggt
     3841 gttaaatggg taactgtgca accatactta ctcatccatt ccgctaaacg gcccggtgta
     3901 ccaatatcat cttgagtagg gacatacaat tgggcaccta aaaataatgg tgtaaacata
     3961 tctctttgaa ttggatcatg tgcaataccg ctcagcattg tgaatttatc attttctgtt
     4021 aagttgaacc ttttggacat ccaattgaaa taataagcca aggaaaaatg tctaccaaga
     4081 acacccttag gaataccttc ggaaccagat gtgaaagata gggttgggtt ggaatctggt
     4141 ccaactacaa cacctgttct ggtgtctttg tagtgatcat atggagccaa aacatcctcg
     4201 ccattgtcca atttgccacc ttcaatggta ccattttctt gaatagcgat ggaattgatt
     4261 cttgaaacaa tctccaattc atcattgatg taatcttcta ctagttgatc caattgtcca
     4321 gcagctctaa taacaatcaa cccacgtggt ttagcaacac ctaagtaaat ggtttgtctg
     4381 gctgggggat atgcagggtc gataactgaa aaggttgcgc cggctttcaa gacacccatc
     4441 acacatacca tcaaatccac acccctagaa gaatagatca tcactacatc acctcttttg
     4501 atacctgttt taatcaaata atgggcaact atgttagaag tgcggttgat gtcgcgataa
     4561 gtgaaagaac gggacttgtc ggaatttagt gttggagtct ccacaacaca ggttctctct
     4621 gggaaggctt cagcattgtc ctggaaaatg tcgtgaatac accccacgaa atcgcaccag
     4681 cccaagttct tagttggatc aggtaaacta tccttggatg atgcggtgat cagagagatt
     4741 ttagttatgc aattggatgg atcgcttagc gcagcagtca aatattgagt aaattggtcc
     4801 gcaacaatgg ttactctttc attcgaatac agtaagctgt tataaattaa gttcaaaaca
     4861 tgcgcattat tactggtatc caaattcaaa gcaaagtcca ctaaatgatg cttgaactcg
     4921 tctaatttga aatcttggtt ttccaaaaag gccaaacgga acaactgagg ggtcctttcc
     4981 agatcttggc aactttgaat tttttcagct agctcgtcaa aggaaaaatt ggcctcaata
     5041 gaattgagct tgttcaactc attgttaatt gtagaataca gctcattaaa tgaccacgtt
     5101 ggttgaatat tgaatcttaa gattttgtta ttcgcaatat aaagaacaat atcatcgtca
     5161 ccggttactc tatatatcaa tgcagcccat acactcaaag cgacagcgta tttgttagaa
     5221 aaactatcat gaggcacatc gagctgaggt agctgtaacg aatatgtagc ttgtttcgta
     5281 taaggttctt gttgtgggcg taaaaagtca tgtggtaaca ctgaaagagt tggattatcc
     5341 aacttctcta tccagacctt ttcgttagtc attagtgagt aactctgtga tatctctcta
     5401 taattagcag tttttcactg aaattccagg aaaggtaata aactcagatt tttttttata
     5461 ctattggctg cttgttactt atatatcttg aacttctccc agcgggtctt caaatacatt
     5521 tgggcgatgt tcatgttcat taggcaggta tttcgacatt gagtcacacg cgaaaaaccg
     5581 ccggaatttt ttatgtaatt gcaagtggaa ttatatgcgg tgtgaaatac cgcacagatg
     5641 cgtaaggaga aaataccgca tcaggaaatt gtaaacgtta atattttgtt aaaattcgcg
     5701 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     5761 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     5821 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     5881 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     5941 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     6001 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     6061 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     6121 tcgcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     6181 tcgctattac gccagctggc gaagggggga tgtgctgcaa ggcgattaag ttgggtaacg
     6241 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgacacata cgatttaggt
     6301 gacactatag aacgcggccg ccagctgaag cttcgtacgc tgcaggtcga cggatccccg
     6361 ggttaattaa ggcgcgccag atctgtttag cttgccttgt ccccgccggg tcacccggcc
     6421 agcgacatgg aggcccagaa taccctcctt gacagtcttg acgtgcgcag ctcaggggca
     6481 tgatgtgact gtcgcccgta catttagccc atacatcccc atgtataatc atttgcatcc
     6541 atacattttg atggccgcac ggcgcgaagc aaaaattacg gctcctcgct gcagacctgc
     6601 gagcagggaa acgctcccct cacagacgcg ttgaattgtc cccacgccgc gcccctgtag
     6661 agaaatataa aaggttagga tttgccactg aggttcttct ttcatatact tccttttaaa
     6721 atcttgctag gatacagttc tcacatcaca tccgaacata aacaaccatg ccacaatttg
     6781 gtatattatg taaaacacca cctaaggtgc ttgttcgtca gtttgtggaa aggtttgaaa
     6841 gaccttcagg tgagaaaata gcattatgtg ctgctgaact aacctattta tgttggatga
     6901 ttacacataa cggaacagca atcaagagag ccacattcat gagctataat actatcataa
     6961 gcaattcgct gagtttcgat attgtcaata aatcactcca gtttaaatac aagacgcaaa
     7021 aagcaacaat tctggaagcc tcattaaaga aattgattcc tgcttgggaa tttacaatta
     7081 ttccttacta tggacaaaaa catcaatctg atatcactga tattgtaagt agtttgcaat
     7141 tacagttcga atcatcggaa gaagcagata agggaaatag ccacagtaaa aaaatgctta
     7201 aagcacttct aagtgagggt gaaagcatct gggagatcac tgagaaaata ctaaattcgt
     7261 ttgagtatac ttcgagattt acaaaaacaa aaactttata ccaattcctc ttcctagcta
     7321 ctttcatcaa ttgtggaaga ttcagcgata ttaagaacgt tgatccgaaa tcatttaaat
     7381 tagtccaaaa taagtatctg ggagtaataa tccagtgttt agtgacagag acaaagacaa
     7441 gcgttagtag gcacatatac ttctttagcg caaggggtag gatcgatcca cttgtatatt
     7501 tggatgaatt tttgaggaat tctgaaccag tcctaaaacg agtaaatagg accggcaatt
     7561 cttcaagcaa taaacaggaa taccaattat taaaagataa cttagtcaga tcgtacaata
     7621 aagctttgaa gaaaaatgcg ccttattcaa tctttgctat aaaaaatggc ccaaaatctc
     7681 tcattggaag acatttgatg acctcatttc tttcaatgaa gggcctaacg gagttgacta
     7741 atgttgtggg aaattggagc gataagcgtg cttctgccgt ggccaggaca acgtatactc
     7801 atcagataac agcaatacct gatcactact tcgcactagt ttctcggtac tatgcatatg
     7861 atccaatatc aaaggaaatg atagcattga aggatgagac taatccaatt gaggagtggc
     7921 agcatataga acagctaaag ggtagtgctg aaggaagcat acgatacccc gcatggaatg
     7981 ggataatatc acaggaggta ctagactacc tttcatccta cataaataga cgcatataag
     8041 tacgcattta agcataaaca cgcactatgc cgttcttctc atgtatatat atatacaggc
     8101 aacacgcaga tataggtgcg acgtgaacag tgagctgtat gtgcgcagct cgcgttgcat
     8161 tttcggaagc gctcgttttc ggaaacgctt tgaagttcct attccgaagt tcctattctc
     8221 tagttctaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt
     8281 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     8341 tcacaattcc acacaacata ggagccggaa gcataaagtg taaagcctgg ggtgcctaat
     8401 gagtgaggta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     8461 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     8521 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     8581 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     8641 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     8701 tggcgttttt ccataggctc ggcccccctg acgagcatca caaaaatcga cgctcaagtc
     8761 agaggtggcg aaacccgaca ggactataaa gataccaggc gttcccccct ggaagctccc
     8821 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8881 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
     8941 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     9001 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     9061 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     9121 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     9181 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     9241 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     9301 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     9361 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     9421 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     9481 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactgc
     9541 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     9601 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     9661 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     9721 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9781 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9841 aacgatcaag gcgagttaca tgatccccca tgttgtgaaa aaaagcggtt agctccttcg
     9901 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9961 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    10021 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    10081 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    10141 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    10201 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    10261 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    10321 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    10381 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cg
//
