LOCUS       sequence-73716-e         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependant TVA receptor-mCherry fusion with a mutation (66T) in 
            TVA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Miyamichi K, Shlomai-Fuchs Y, Shu M, Weissbourd BC, Luo L, Mizrahi A
  TITLE     Dissecting local circuits: parvalbumin interneurons underlie broad 
            feedback control of olfactory bulb output.
  JOURNAL   Neuron. 2013 Dec 4;80(5):1232-45. doi: 10.1016/j.neuron.2013.08.027.
            Epub 2013 Nov 14.
  PUBMED    24239125
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.08.027"; journalName: "Neuron"; date: 
            "2013-12-4- 4"; volume: "80"; issue: "5"; pages: "1232-45"
COMMENT     Sequence Label: CAG-Flex-TC66T
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    80..113
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    184..217
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      224..233
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 anacatagtt agataccagt caatctttca caaattttgt aatccagagg ttgattatcg
       61 atcggccgca tataagctta taacttcgta taatgtatgc tatacgaagt tatgggtcga
      121 tggtgagatc tggactagag ggtcgatggt gatgcttgga tcaaaatagg aagaatccca
      181 atgataactt cgtataaagt atcctatacg aagttatagg cgcgccacca tggcgcggct
      241 gctgcccgcg ctgctgctgc tgctgctgcc cggtaacgtg accggtaacg ggtccggtaa
      301 cggttctttg tcccgttgcc cccccggtca gttccgctgc tcggagccgc ccggtgccca
      361 cggggagtgt tacccgcagg actggctgtg cgacggacac cccgactgcg acgacgggcg
      421 ggacacctgg ggctgcggga ccagcgcgat ccccgcggtg cccacggaca acggcacaga
      481 ggctcccact gcccctgctc ctggacgtgc tctgccagcc aggaatcacg gccgcatgtg
      541 gatgctgatc actgcagtgc tcctgtgctg cctggtagct gtgggtggta tcgctgcatg
      601 ggggaagtcc aaagcaaaaa gcaggtctga catcttcagt cttgaaagcg catccaaggg
      661 gggtggggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
      721 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
      781 gggccgcccc tacgagggca cccanaccgc caagctgaag tgaccaaagg gtggccccct
      841 gcccttccct ggncatcctg tcccctcatt tcatgtacgg ctccnngcct accngaagca
      901 ccccgncgac atccccgact actttnaann tgttctttcn nnnggctnca gtnnnncgcg
      961 tgatgaactt nnnnangacg nnnngntgac nnnna
//
