LOCUS       sequence-73717-e         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependant TVA receptor-mCherry fusion with a mutation (66T) in 
            TVA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Miyamichi K, Shlomai-Fuchs Y, Shu M, Weissbourd BC, Luo L, Mizrahi A
  TITLE     Dissecting local circuits: parvalbumin interneurons underlie broad 
            feedback control of olfactory bulb output.
  JOURNAL   Neuron. 2013 Dec 4;80(5):1232-45. doi: 10.1016/j.neuron.2013.08.027.
            Epub 2013 Nov 14.
  PUBMED    24239125
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.08.027"; journalName: "Neuron"; date: 
            "2013-12-4- 4"; volume: "80"; issue: "5"; pages: "1232-45"
COMMENT     Sequence Label: CAG-Flex-TC66T
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    24..57
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    120..153
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(160..867)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSXGEEDNMAIIXEFMRFKVHMEGSVNGHXFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(438..457)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     707..725
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 atgntaccgc gggcccggcc gggataactt cgtataaagt atcctatacg aagttatatc
       61 aaaataggaa gaccaatgct tcaccatcga cccgaattgc caagcatcac catcgacaca
      121 taacttcgta taatgtatgc tatacgaagt tatgtcgact tacttgtaca gctcgtccat
      181 gccgccggtg gagtggcggc cctcggcgcg ttcgtactgt tccacgatgg tgtagtcctc
      241 gttgtgggag gtgatgtcca acttgatgtt gacgttgtag gcgccgggca gctgcacggg
      301 cttcttggcc ttgtaggtgg tcttgacctc agcgtcgtag tggccgccgt ccttcagctt
      361 cagcctctgc ttgatctcgc ccttcagggc gccgtcctcg gggtacatcc gctcggagga
      421 ggcctcccag cccatggtct tcttctgcat tacggggccg tcggagggga agttggtgcc
      481 gcgcagcttc accttgtaga tgaactcgcc gtcctgcagg gaggagtcct gggtcacggt
      541 caccacgccg ccgtcctcga agttcatcac gcgctcccac ttgaagccct cggggaagga
      601 cagcttcaag tagtcgggga tgtcggcggg gtgcttcacg tangccttgg agccgtacat
      661 gaactgaggg gacaggatgt cccaggcgaa gggcaggggg ccacccttgg tcaccttcag
      721 cttggcggtc tgggtgccct cgtaggggcg gccctcgccc tcgccctcga tctcgaantc
      781 gtggccgttc acggagccct ccatgtgcac cttgaagcgc atgaactccn tgatgatggc
      841 catgttatcc tcctcgcccn ngctcacccc cacccccctt nnngcncttt canantggaa
      901 gaatgtcaga ctgctttttg ctttgaactt ccccntggca gcganncncn cnnagnctnc
      961 cangcagccn nggagnannn nnnnnntngn nnntc
//
